Baculovirus Expression System with Gateway™ Technology
본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기
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인용 및 참조 문헌 (3)
Invitrogen™

Baculovirus Expression System with Gateway™ Technology

The Baculovirus Expression System with Gateway™ Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus자세히 알아보기
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카탈로그 번호수량
1182701120 reactions
카탈로그 번호 11827011
제품 가격(KRW)
2,431,000
Online offer
Ends: 31-Dec-2025
2,859,000
할인액 428,000 (15%)
Each
카트에 추가하기
수량:
20 reactions
제품 가격(KRW)
2,431,000
Online offer
Ends: 31-Dec-2025
2,859,000
할인액 428,000 (15%)
Each
카트에 추가하기
The Baculovirus Expression System with Gateway™ Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus Expression System with Gateway™ Technology offers:

• Expression using the Bac-to-Bac™ technology (1)
• Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
• pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)
For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형Expression System
수량20 reactions
벡터pDEST, Gateway Baculovirus Vectors
클로닝 방법Gateway
제품라인Gateway
프로모터Polyhedrin
단백질 태그GST Tag, His Tag (6x), Untagged
Unit SizeEach
구성 및 보관
The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Baculovirus Expression System with Gateway™ Technology FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.
Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,
Journal:J Biol Chem
PubMed ID:14699162
'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More
Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.
Authors:Suzuki T, Okada Y, Semba S, Orba Y, Yamanouchi S, Endo S, Tanaka S, Fujita T, Kuroda S, Nagashima K, Sawa H,
Journal:J Biol Chem
PubMed ID:15843383
'The human polyomavirus JC virus (JCV) is the causative agent of a fatal demyelinating disease, progressive multifocal leukoencephalopathy, and encodes six major proteins, including agnoprotein. Agnoprotein colocalizes with microtubules in JCV-infected cells, but its function is not fully understood. We have now identified fasciculation and elongation protein zeta 1 (FEZ1) ... More
A novel abetalipoproteinemia genotype. Identification of a missense mutation in the 97-kDa subunit of the microsomal triglyceride transfer protein that prevents complex formation with protein disulfide isomerase.
Authors: Rehberg E F; Samson-Bouma M E; Kienzle B; Blinderman L; Jamil H; Wetterau J R; Aggerbeck L P; Gordon D A;
Journal:J Biol Chem
PubMed ID:8939939
The microsomal triglyceride transfer protein (MTP) is a heterodimer composed of the ubiquitous multifunctional protein, protein disulfide isomerase, and a unique 97-kDa subunit. Mutations that lead to the absence of a functional 97-kDa subunit cause abetalipoproteinemia, an autosomal recessive disease characterized by a defect in the assembly and secretion of ... More