RPMI 1640 Medium
RPMI 1640 Medium
인용 및 참조 문헌 (44)
Gibco™

RPMI 1640 Medium

RPMI 1640 Medium은 원래 인간 백혈병 세포의 단일층(monolayer) 혹은 현탁(suspension) 배양을 위해 개발된 제품입니다. Roswell Park Memorial Institute (RPMI) 1640자세히 알아보기
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카탈로그 번호수량
11875101100 mL
1187512720 x 100 mL
11875093500mL
1187511910 x 500mL
118750851000mL
118751356 x 1000mL
카탈로그 번호 11875101
제품 가격(KRW)
28,000
Online offer
Ends: 31-Dec-2025
29,000
할인액 1,000 (3%)
Each
카트에 추가하기
수량:
100 mL
Customize this product
제품 가격(KRW)
28,000
Online offer
Ends: 31-Dec-2025
29,000
할인액 1,000 (3%)
Each
카트에 추가하기
RPMI 1640 Medium은 원래 인간 백혈병 세포의 단일층(monolayer) 혹은 현탁(suspension) 배양을 위해 개발된 제품입니다. Roswell Park Memorial Institute (RPMI) 1640 Medium은 HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas 등의 다양한 포유류 세포에 적합한 것으로 확인되었습니다. 당사는 광범위한 세포 배양 응용 분야에 따라 다양하게 조성이 변경된 RPMI 1640 Medium를 제공합니다. 배지 선택 도구를 사용하여 적절한 조성을 간편하게 찾을 수 있습니다.

이 RPMI은 다음과 같이 조성이 변경되었습니다.
포함  제외 성분
• L-글루타민  • HEPES
• 페놀 레드 

모든 조성을 이용할 수 있습니다.

RPMI의 사용
RPMI1640 배지는 다른 배지에 비해 특징적으로 환원제 글루타티온과 고농도의 비타민을 함유하고 있습니다. RPMI 1640 medium에는 Eagle's Minimal Essential Medium이나 Dulbecco's Modified Eagle Medium에는 없는 biotin, vitamin B12, PABA가 포함되어 있습니다. 또한 비타민 이노시톨과 콜린도 매우 높은 농도로 함유되어 있습니다. RPMI 1640 Medium에는 단백질, 지질, 성장인자가 들어있지 않습니다. 따라서 RPMI 1640 배지는 일반적으로 10% 태아 소 혈청(FBS)을 보충해야 합니다. RPMI 1640 배지는 중탄산나트륨(sodium bicarbonate) 버퍼 시스템(2.0 g/L)을 이용하며, 이로 인해 생리학적인 pH를 유지하기 위해 5–10% CO2 환경이 요구됩니다.

cGMP 제조 및 품질 시스템
RPMI 1640 배지는 뉴욕주의 Grand Island에 위치한 cGMP 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다. 공급망 유지를 위해 당사는 당사의 스코틀랜드 시설에서도 동일한 RPMI 1640 제품을 제공합니다(21875-158). 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes 및 carcinomas
세포 유형백혈병 세포
농도1X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
수량100 mL
유통 기한제조일로부터 12개월
배송 조건실온
분류동물 원료 성분 비함유
형태액체
Serum LevelStandard Serum Supplementation
멸균멸균 여과
Sterilization MethodSterile-filtered
첨가제 포함L-글루타민, 페놀 레드, Phenol Red
첨가제 없음HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
보관 조건: 2∼8°C. 차광 보관
배송 조건: 상온
유통 기한: 제조일로부터 12개월

자주 묻는 질문(FAQ)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all RPMI 1640 Medium FAQs

인용 및 참조 문헌 (44)

인용 및 참조 문헌
Abstract
Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells.
Authors:Wang Qin; Liu Min; Mullah Bashar; Siderovski David P; Neubig Richard R;
Journal:J Biol Chem
PubMed ID:12006602
Regulators of G protein signaling (RGS) proteins compose a highly diverse protein family best known for inhibition of G protein signaling by enhancing GTP hydrolysis by Galpha subunits. Little is known about the function of endogenous RGS proteins. In this study, we used synthetic ribozymes targeted to RGS2, RGS3, RGS5, ... More
Silencing of Transcription of the Human Luteinizing Hormone Receptor Gene by Histone Deacetylase-mSin3A Complex.
Authors: Zhang Ying; Dufau Maria L;
Journal:J Biol Chem
PubMed ID:12091390
'Modification of chromatin structure by histone acetylases and deacetylases is an important mechanism in modulation of eukaryotic gene transcription. The present study investigated regulation of the human luteinizing hormone receptor (hLHR) gene by histone deacetylases. Inhibition of histone deacetylases (HDACs) by trichostatin A (TSA) increased hLHR promoter activity by 40-fold ... More
Distinct Intracellular Signaling in Tumor Necrosis Factor-related Apoptosis-inducing Ligand- and CD95 Ligand-mediated Apoptosis.
Authors: Velthuis Jurjen H L; Rouschop Kasper M A; De Bont Hans J G M; Mulder Gerard J; Nagelkerke J Fred;
Journal:J Biol Chem
PubMed ID:11980895
'Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in tumor cells but not in healthy cells. Similar to CD95 ligand (CD95L), TRAIL signaling requires ligand-receptor interaction; the downstream signaling molecules, such as Fas-associated death domain and caspase-8, also seem similar. Using cells stably expressing TRAIL and ... More
Intercellular calcium signaling occurs between human osteoblasts and osteoclasts and requires activation of osteoclast P2X7 receptors.
Authors: Jørgensen Niklas R; Henriksen Zanne; Sørensen Ole H; Eriksen Erik F; Civitelli Roberto; Steinberg Thomas H;
Journal:J Biol Chem
PubMed ID:11756404
'Signaling between osteoblasts and osteoclasts is important in bone homeostasis. We previously showed that human osteoblasts propagate intercellular calcium signals via two mechanisms: autocrine activation of P2Y receptors, and gap junctional communication. In the current work we identified mechanically induced intercellular calcium signaling between osteoblasts and osteoclasts and among osteoclasts. ... More
Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. Role of caspase-3.
Authors: Rehm Markus; Dussmann Heiko; Janicke Reiner U; Tavare Jeremy M; Kogel Donat; Prehn Jochen H M;
Journal:J Biol Chem
PubMed ID:11964393
'Activation of effector caspases is considered to be the final step in many apoptosis pathways. We transfected HeLa cells with a recombinant caspase substrate composed of cyan and yellow fluorescent protein and a linker peptide containing the caspase cleavage sequence DEVD, and we examined the cleavage kinetics at the single-cell ... More
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