SuperScript V VILO Master Mix, Primer-Free, with gDNA Removal, 500 rxns

Invitrogen™ SuperScript™ V VILO™ Master Mix, Primer-Free is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis with the flexibility to select your preferred priming strategy. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix, Primer-Free is also available with ezDNase™ Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix, Primer-Free include:

• Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
• Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
• Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
• Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
• Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix, Primer-Free combines SuperScript V RT, RNase inhibitor, buffer, and dNTPs in a single formulation while allowing users to select and optimize primers for their specific application. This flexibility supports gene-specific primers, oligo(dT), random primers, or customized primer combinations while maintaining the convenience of a ready-to-use master mix.

SuperScript V VILO Master Mix, Primer-Free with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA.

SuperScript V VILO Master Mix, Primer-Free also features a DTT-free, animal origin–free formulation for improved sustainability and simplified reagent handling.