PureLink™ DNase Set

The PureLink™ DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink™ RNA자세히 알아보기

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12185010	50 Preps

카탈로그 번호 12185010

제품 가격(KRW)

177,000

온라인 행사

Ends: 31-Dec-2025

196,000

할인액 19,000 (10%)

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50 Preps

제품 가격(KRW)

177,000

온라인 행사

Ends: 31-Dec-2025

196,000

할인액 19,000 (10%)

카트에 추가하기

The PureLink™ DNase Set provides rapid and efficient removal of DNA from RNA that has been purified using PureLink™ RNA kits. The PureLink™ DNase Set is:

• RNase-free for use in RNA purification
• Optimized for on-column digestion
• Lyophilized for stability

Using the PureLink™ DNase Set
The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink™ protocols. The degree of DNA digestion performed by PureLink™ DNase is optional for most PureLink™ RNA kits, due to the efficient removal of the majority of DNA by the PureLink™ technology; however, some downstream applications that are sensitive to small amounts of DNA may require further removal of DNA.

For Research Use Only. Not for use in diagnostic procedures.

농도10X

반응 수50 Preps

수량50 Preps

배송 조건Room Temperature

Unit SizeEach

구성 및 보관

Contains:
• PureLink™ DNase (1500 U, lyophilized)
• 10X DNase I Reaction Buffer (1 mL)
• RNase-Free Water (1 mL)

Store at 4°C upon receipt. After reconstituted, PureLink™ DNase should be stored at -20°C.

자주 묻는 질문(FAQ)

How can I inactivate DNase I?

Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 molar ratio of Mg++ ions:EDTA) to chelate the Mg++ ions in the DNase I buffer. Heat for 10 min at 65 degrees C.

Note: It is vital that the EDTA be at least at 2 mM prior to heat-inactivation to avoid Mg-dependent RNA hydrolysis.

DNA-free and Turbo-free versions of DNase I can be inactivated with included DNase Inactivation Reagent.

Are your DNase I products RNase-free?

Most of our DNase I products are guaranteed free of RNase activity. However, please note that Cat. No. 18047-019 is not tested for RNAse and is recommended primarily for protein applications. The other products are suitable for removing DNA from both RNA and protein preparations, for nick translating DNA, and for generating random fragments of DNA. For more demanding RT-PCR applications, we recommend using DNAse I, Amplification Grade.