Oligofectamine™ Transfection Reagent
Oligofectamine™ Transfection Reagent
인용 및 참조 문헌 (48)
Invitrogen™

Oligofectamine™ Transfection Reagent

Oligofectamine™ Transfection Reagent는 안정된 oligos complex를 형성해 높은 특이성과 비독성으로 진핵세포 형질 감염 효율을 높입니다.Oligofectamine™ 는 비특이적 영향이 적은 높은자세히 알아보기
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카탈로그 번호수량
122520111 mL
카탈로그 번호 12252011
제품 가격(KRW)
734,000
Each
카트에 추가하기
수량:
1 mL
제품 가격(KRW)
734,000
Each
카트에 추가하기
Oligofectamine™ Transfection Reagent는 안정된 oligos complex를 형성해 높은 특이성과 비독성으로 진핵세포 형질 감염 효율을 높입니다.

Oligofectamine™ 는 비특이적 영향이 적은 높은 특이 활성(표적 유전자 knockdown)과 전달 기전 결과로 뛰어난 성능을 제공합니다(그림 1). Oligofectamine™는 핵과 세포혈장 표적에 적합하며 CHO, HEK-293, NIH 3T3, HeLa 등 다양한 cell line의 형질 감염에 사용됩니다.

Oligofectamine™ Reagent은 간편하고 신속한 프로토콜을 제공하여 사용이 용이합니다. Oligofectamine™ Reagent를 희석해 oligonucleotide와 섞은 후 실험 세포를 추가하기만 하세요. Oligofectamine™는 귀한 oligonucleotide 필요양을 수천 배까지 줄여 매우 적은 나노몰 수준의 antisense oligonucleotide만 필요로 합니다. 이런 특성으로 처리율이 높은 어플리케이션에 적용이 이상적입니다.

또한 Oligofectamine™는 siRNA transfections에도 효과적입니다. HeLa 세포에서 RNAi knockdown 실험을 실시할 때 이 시약을 추천합니다. 자세한 정보는 RNAi Central을 방문하세요.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
고처리량 호환성High-throughput Compatible
제품라인Oligofectamine
제품 유형Transfection Reagent
수량1 mL
혈청 호환 가능No
배송 조건Wet Ice
셀 유형Established Cell Lines, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Synthetic siRNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Contains one vial (1 ml) Oligofectamine™ Reagent. Store at 4°C. Do not freeze.

자주 묻는 질문(FAQ)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all Oligofectamine™ Transfection Reagent FAQs

인용 및 참조 문헌 (48)

인용 및 참조 문헌
Abstract
A high-throughput, cell-based screening method for siRNA and small molecule inhibitors of mTORC1 signaling using the In Cell Western technique.
Authors:Hoffman GR, Moerke NJ, Hsia M, Shamu CE, Blenis J,
Journal:Assay Drug Dev Technol
PubMed ID:20085456
'The mTORC1 pathway is a central regulator of cell growth, and defective mTORC1 regulation plays a causative role in a variety of human diseases, including cancer, tumor syndromes such as the tuberous sclerosis complex (TSC) and lymphangioleiomyomatosis (LAM), and metabolic diseases such as diabetes and obesity. Given the importance of ... More
Processing of Pro-atrial Natriuretic Peptide by Corin in Cardiac Myocytes.
Authors: Wu Faye; Yan Wei; Pan Junliang; Morser John; Wu Qingyu;
Journal:J Biol Chem
PubMed ID:11884416
'Corin is a type II transmembrane serine protease abundantly expressed in the heart. In a previous study using transfected 293 cells, we showed that corin converted pro-atrial natriuretic peptide (pro-ANP) to atrial natriuretic peptide (ANP), suggesting that corin is likely the pro-ANP convertase. Because other serine proteases such as thrombin ... More
Systems survey of endocytosis by multiparametric image analysis.
Authors:Collinet C, Stöter M, Bradshaw CR, Samusik N, Rink JC, Kenski D, Habermann B, Buchholz F, Henschel R, Mueller MS, Nagel WE, Fava E, Kalaidzidis Y, Zerial M,
Journal:Nature
PubMed ID:20190736
'Endocytosis is a complex process fulfilling many cellular and developmental functions. Understanding how it is regulated and integrated with other cellular processes requires a comprehensive analysis of its molecular constituents and general design principles. Here, we developed a new strategy to phenotypically profile the human genome with respect to transferrin ... More
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
Authors:Glozman R, Okiyoneda T, Mulvihill CM, Rini JM, Barriere H, Lukacs GL,
Journal:J Cell Biol
PubMed ID:19307599
'N-glycosylation, a common cotranslational modification, is thought to be critical for plasma membrane expression of glycoproteins by enhancing protein folding, trafficking, and stability through targeting them to the ER folding cycles via lectin-like chaperones. In this study, we show that N-glycans, specifically core glycans, enhance the productive folding and conformational ... More
Disinhibition of neurotrophin-induced dorsal root ganglion cell neurite outgrowth on CNS myelin by siRNA-mediated knockdown of NgR, p75(NTR) and Rho-A.
Authors:Ahmed Z, Dent RG, Suggate EL, Barrett LB, Seabright RJ, Berry M, Logan A,
Journal:Mol Cell Neurosci
PubMed ID:15737741
'The presence of multiple axon growth inhibitors may partly explain why central nervous system axons are generally incapable of regenerating after injury. Using RNA interference (RNAi) in dorsal root ganglia neurons (DRGN), we demonstrate siRNA-mediated silencing of components of the inhibitory signalling cascade, including p75(NTR), NgR and Rho-A mRNA, of ... More
View all Oligofectamine™ Transfection Reagent citations