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인용 및 참조 문헌 (3)
Invitrogen™
Gateway™ pET-DEST42 Vector
The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 12276010 | 6 μg |
카탈로그 번호 12276010
제품 가격(KRW)
613,000
온라인 행사
Ends: 31-Mar-2026
700,000할인액 87,000 (12%)
Each
수량:
6 μg
제품 가격(KRW)
613,000
온라인 행사
Ends: 31-Mar-2026
700,000할인액 87,000 (12%)
Each
The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:
• Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway™ entry vector
• Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway™ entry vector
For Research Use Only. Not for use in diagnostic procedures.
사양
항생제 내성 박테리아Ampicillin (AmpR)
구성 또는 유도성 시스템Inducible
유도제IPTG
제품 유형Gateway System Destination Expression Vector
수량6 μg
선택 제제(진핵)None
벡터pET, pDEST
클로닝 방법Gateway
제품라인Gateway
프로모터T7, lacO
단백질 태그His Tag (6x), V5 Epitope Tag
Unit SizeEach
구성 및 보관
The Champion™ pET-DEST42 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.
자주 묻는 질문(FAQ)
Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?
Do you have a recommended single-step protocol for BP/LR recombination?
How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?
Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?
Do you offer Gateway vectors for expression in plants?
인용 및 참조 문헌 (3)
인용 및 참조 문헌
Abstract
Engineering vitamin E content: from Arabidopsis mutant to soy oil.
Journal:Plant Cell
PubMed ID:14630966
'We report the identification and biotechnological utility of a plant gene encoding the tocopherol (vitamin E) biosynthetic enzyme 2-methyl-6-phytylbenzoquinol methyltransferase. This gene was identified by map-based cloning of the Arabidopsis mutation vitamin E pathway gene3-1 (vte3-1), which causes increased accumulation of delta-tocopherol and decreased gamma-tocopherol in the seed. Enzyme assays
bZIP transcription factor interactions regulate DIF responses in Dictyostelium.
Journal:Development
PubMed ID:16410410
The signalling molecule DIF-1 is required for normal cell fate choice and patterning in Dictyostelium. To understand how these developmental processes are regulated will require knowledge of how cells receive and respond to the DIF-1 signal. Previously, we have described a bZIP transcription factor, DimA, which is required for cells
Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family.
Journal:Eur J Biochem
PubMed ID:15373841
The Mycobacterium tuberculosis genome contains an unusually high number of proteins involved in the metabolism of lipids belonging to the Lip family, including various nonlipolytic and lipolytic hydrolases. Driven by a structural genomic approach, we have biochemically characterized the Rv1399c gene product, LipH, previously annotated as a putative lipase. Rv1399c