Gateway™ pT-Rex™-DEST31 Vector
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인용 및 참조 문헌 (1)
Invitrogen™

Gateway™ pT-Rex™-DEST31 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,자세히 알아보기
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카탈로그 번호수량
12302014
12302-014으로도 사용됨
6 μg
카탈로그 번호 12302014
12302-014으로도 사용됨
제품 가격(KRW)
-
수량:
6 μg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Inducible
배달 유형Transfection
용도(애플리케이션)Regulated Expression
유도제Tetracycline
제품 유형Gateway System Destination Expression Vector
수량6 μg
선택 제제(진핵)Geneticin™ (G-418)
벡터pDEST
클로닝 방법Gateway
제품라인Gateway, T-REx
프로모터CMV/TO
단백질 태그His Tag (6x)
Unit Size6 µg
구성 및 보관
All destination vectors are provided lyophilized and supercoiled.

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pT-Rex™-DEST31 Vector FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Dual-tagging system for the affinity purification of mammalian protein complexes.
Authors:Giannone RJ, McDonald WH, Hurst GB, Huang Y, Wu J, Liu Y, Wang Y,
Journal:Biotechniques
PubMed ID:17907572
'Although affinity purification coupled with mass spectrometry (MS) provides a powerful tool to study protein-protein interactions, this strategy has encountered numerous difficulties when adapted to mammalian cells. Here we describe a Gateway-compatible dual-tag affinity purification system that integrates regulatable expression, tetracysteine motifs, and various combinations ofaffinity tags to facilitate the ... More