OptiPRO™ SFM
OptiPRO™ SFM
Gibco™

OptiPRO™ SFM

OptiPRO™ SFM는 바이러스 및 재조합 단백질 생성에 중요한 COS-7, MDBK, HeLa, MDCK, PK-15, VERO, BHK-21 등 여러 가지 신장 파생자세히 알아보기
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카탈로그 번호수량
12309050100 mL
123090191000 mL
카탈로그 번호 12309050
제품 가격(KRW)
-
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수량:
100 mL
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OptiPRO™ SFM는 바이러스 및 재조합 단백질 생성에 중요한 COS-7, MDBK, HeLa, MDCK, PK-15, VERO, BHK-21 등 여러 가지 신장 파생 cell line 성장을 위해 만들어진 무혈청 배지로 동물 기원 성분을 함유하고 있지 않습니다.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주HeLa, MDCK, BHK-21, PK-15, COS-7, MDBK, VERO
농도1 X
엔도톡신 수준Low
형식Bottle(s)
항생제 포함No antibiotics
제품라인Gibco
제품 유형Serum Free Medium
수량100 mL
배송 조건Room Temperature
분류Animal Origin-free, Low Protein, Serum-free
Culture TypeAdherent Cell Culture
형태Liquid
Serum LevelSerum-free
첨가제 포함Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine
첨가제 없음No Glutamine
Unit SizeEach
구성 및 보관
Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

자주 묻는 질문(FAQ)

Do you offer a protocol for titering virus in OptiPRO SFM?

For virus titering in OptiPRO SFM we recommend using Falcon or equivalent 96-well plates and plating cells at a concentration of 1 X 10^6/mL. If you are having trouble growing cells for virus titration in OptiPRO SFM in the 96-well plates, we suggest switching to the EMEM with 5%FBS or DMEM with 5% FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a recommended protocol for culturing cell lines in OptiPRO SFM that require sequential adaptation?

The sequential adaptation process is a multi-subculture of cells. We recommend the following passaging sequence:
• First passage: use 25% OptiPRO SFM:75% original stock medium
• Second passage: use 50% OptiPRO SFM:50% original stock medium
• Third passage: use 75% OptiPRO SFM:25% original stock medium
• Fourth and subsequent passages: use 100% OptiPRO SFM

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I add antibiotics to OptiPRO SFM?

You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What concentration of L-glutamine or GlutaMAX-I should I add to OptiPRO SFM before I use it?

We recommend adding L-glutamine or GlutaMAX-I to OptiPRO SFM at a final concentration of 4mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.