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인용 및 참조 문헌 (2)
Gibco™
McCoy's 5A (Modified) Medium, HEPES
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 12330031 | 500 mL |
카탈로그 번호 12330031
제품 가격(KRW)
81,000
Online offer
Ends: 31-Mar-2026
89,000할인액 8,000 (9%)
Each
수량:
500 mL
제품 가격(KRW)
81,000
Online offer
Ends: 31-Mar-2026
89,000할인액 8,000 (9%)
Each
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
The complete formulation is available.
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ McCoy's 5A (modified) is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ McCoy's 5A (modified) product made in our Scotland facility (22300-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This product is not used for in vitro diagnostic purpose in some countries.
This McCoy's 5A is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | |
| • Bacto-peptone | |
| • Phenol Red | |
| • HEPES |
The complete formulation is available.
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ McCoy's 5A (modified) is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ McCoy's 5A (modified) product made in our Scotland facility (22300-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
This product is not used for in vitro diagnostic purpose in some countries.
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For human ex vivo tissue and cell culture processing applications.
사양
세포주Rat fibroblasts
세포 유형Biopsy Tissues
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형McCoy's 5A Modified Medium
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin
형태Liquid
멸균Sterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES, Phenol Red
첨가제 없음No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
How can I remove mycoplasma contamination from my cell culture medium?
I see a decrease in growth of my culture. What should I do?
My cells are not adhering to the culture vessel. What should I do?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
A potential H-DNA element in the MUC1 promoter does not influence transcription.
Journal:J Biol Chem
PubMed ID:8900124
A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact,
B-Raf is dispensable for K-Ras-mediated oncogenesis in human cancer cells.
Journal:Cancer Res
PubMed ID:15026326
Oncogenic mutations in B-Raf and Kirsten-Ras (K-Ras) are mutually exclusive during human cancer pathogenesis. In an effort to study the biological basis of this epistasis, gene targeting was used to create isogenic sets of human cancer cells differing only in presence or absence of endogenous oncogenic K-Ras or wild-type B-Raf.