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인용 및 참조 문헌 (2)
Invitrogen™
One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells
One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 12331013 | 21 x 50 μL |
카탈로그 번호 12331013
제품 가격(KRW)
353,000
Online offer
Ends: 31-Dec-2025
392,000할인액 39,000 (10%)
Each
수량:
21 x 50 μL
제품 가격(KRW)
353,000
Online offer
Ends: 31-Dec-2025
392,000할인액 39,000 (10%)
Each
One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection. The inclusion of the tonA genotype offers added security to protect valuable clones and libraries. MAX Efficiency DH10B T1 cells can achieve high transformation efficiency of >1 x 109 cfu/μg supercoiled plasmid, making them ideal for generating cDNA or genomic libraries, transforming large plasmids (>150 kb in size), and for situations when DNA is limiting.
MAX Efficiency DH10B T1 Phage-Resistant Cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr), allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has the lacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B-T1R cells an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
MAX Efficiency DH10B T1 Phage-Resistant Cells offer:
• Transformation efficiencies of >1 x 109 cfu/μg
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• mcrA genotypic marker and the mcrBC, mrr deletion that allows for cloning DNA that contains methylcytosine and methyladenine
• endA1 mutation that enables increased plasmid yield and quantity
Convenient and efficient format
DH10B-T1R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles, or money wasted on unused cells.
Genotype
F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA
Find the strain and format that fit your needs
We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
The DH10B-T1R strain is available in Multishot format for high throughput applications.
Explore bacterial growth media formats.
For Research Use Only. Not for use in diagnostic procedures.
사양
항생제 내성 박테리아Yes (Streptomycin)
블루/화이트 스크리닝Yes (lacZΔM15)
메틸화 DNA 클로닝Yes (mcrA)
불안정 DNA 클로닝Not suitable for cloning unstable DNA
에프에피솜 포함No
고처리량 호환성Low
플라스미드 품질 개선Yes (endA1)
플라스미드High Copy Plasmid
비메틸화 DNA 준비No
제품라인One Shot
제품 유형Chemically Competent Cells
수량21 x 50 μL
재조합 감소Yes (recA1)
배송 조건Dry Ice
T1 Phage - 저항성(tonA)Yes
형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)
형식Tube
종E. coli (K12)
Unit SizeEach
구성 및 보관
• One Shot MAX Efficiency DH10B T1 Phage-Resistant Cells (21 x 50 μL)
Store Competent Cells at –80°C.
• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.
• S.O.C. Medium (6 ml)
Store S.O.C. Medium at 4°C or room temperature.
.
Store Competent Cells at –80°C.
• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.
• S.O.C. Medium (6 ml)
Store S.O.C. Medium at 4°C or room temperature.
.
자주 묻는 질문(FAQ)
You offer competent cells in Subcloning Efficiency, Library Efficiency and MAX Efficiency. How do these differ?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
Calsarcin-3, a novel skeletal muscle-specific member of the calsarcin family, interacts with multiple Z-disc proteins.
Journal:J Biol Chem
PubMed ID:11842093
'The Z-disc is a highly specialized multiprotein complex of striated muscles that serves as the interface of the sarcomere and the cytoskeleton. In addition to its role in muscle contraction, its juxtaposition to the plasma membrane suggests additional functions of the Z-disc in sensing and transmitting external and internal signals.
Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA.
Journal:BMC Biotechnol
PubMed ID:12546706
BACKGROUND: The ability to manipulate the genetic networks underlying the physiological and behavioural repertoires of the adult honeybee worker (Apis mellifera) is likely to deepen our understanding of issues such as learning and memory generation, ageing, and the regulatory anatomy of social systems in proximate as well as evolutionary terms.