AccuPrime™ GC-Rich DNA Polymerase

Invitrogen™

AccuPrime™ GC-Rich DNA Polymerase

Name: AccuPrime™ GC-Rich DNA Polymerase
SKU: 12337024

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

보기 방식 변경

카탈로그 번호	반응 수
12337024	1000 Reactions
12337016	200 Reactions

2 개 옵션

카탈로그 번호 12337024

제품 가격(KRW)

반응 수:

1000 Reactions

대량 주문 또는 맞춤형 요청

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

AccuPrime GC-Rich DNA Polymerase features

High yields for targets up to 5 kb in length
AccuPrime accessory proteins for improved PCR specificity
Sensitivity down to 5 ng of template DNA

Robust and specific amplifications

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition

One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.

For Research Use Only. Not for use in diagnostic procedures.

사양

Fidelity (Taq 대비)2X

핫 스타트Built-In Hot Start

반응 수1000 Reactions

오버행Blunt

중합효소AccuPrime GC-Rich DNA Polymerase

수량1,000 reactions

반응 형식Separate Components

배송 조건Wet or Dry Ice

크기(최종 제품)5 kb or less

부피500 μL

용도(애플리케이션)Hot-start PCR

GC-Rich PCR PerformanceHigh

반응 속도Standard

Unit SizeEach

구성 및 보관

• AccuPrime GC-Rich DNA Polymerase (1 x 500 μL at 2 U/μL)
• 5X AccuPrime GC-Rich Buffer A (1 x 5 mL)
• 5X AccuPrime GC-Rich Buffer B (1 x 5 mL)
• 50 mM MgSO₄ (1 x 5 mL)

Store at –20°C.

자주 묻는 질문(FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all AccuPrime™ GC-Rich DNA Polymerase FAQs