AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
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12344032
1000 Reactions
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200 Reactions
2 개 옵션
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제품 가격(KRW)
2,670,000
キャンペーン価格
Ends: 30-Sep-2026
3,141,000
할인액 471,000 (15%)
Each
반응 수:
1000 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
2,670,000
キャンペーン価格
Ends: 30-Sep-2026
3,141,000
할인액 471,000 (15%)
Each
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
Greater than 26 times higher fidelity than Taq DNA polymerase
Amplification of fragments up to 12 kb
Minimized PCR optimization
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates
RT-PCR
High-fidelity PCR
Hot-start PCR
Notes
AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)26X
핫 스타트Built-In Hot Start
반응 수1000 Reactions
오버행Blunt
중합효소AccuPrime Pfx DNA Polymerase
수량1,000 reactions
반응 형식Separate Components
배송 조건Wet or Dry Ice
크기(최종 제품)12 kb or less
부피500 μL
검출 방법Primer-probe
용도(애플리케이션)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceLow
반응 속도Standard
Unit SizeEach
구성 및 보관
• AccuPrime Pfx DNA Polymerase (1 x 500 μL at 2.5 U/μL) • 10X AccuPrime Pfx Reaction Mix (5 x 1 mL) • 50 mM MgSO4 (2 x 1 mL)