AccuPrime&trade; <i>Pfx</i> DNA Polymerase
Invitrogen™

AccuPrime™ Pfx DNA Polymerase

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호반응 수
123440321000 Reactions
12344024200 Reactions
카탈로그 번호 12344032
제품 가격(KRW)
2,670,000
キャンペーン価格
Ends: 30-Sep-2026
3,141,000
할인액 471,000 (15%)
Each
반응 수:
1000 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
2,670,000
キャンペーン価格
Ends: 30-Sep-2026
3,141,000
할인액 471,000 (15%)
Each
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

Benefits of AccuPrime Pfx DNA Polymerase

  • Greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 12 kb
  • Minimized PCR optimization

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • High-fidelity PCR
  • Hot-start PCR

Notes

  • AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
  • The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)26X
핫 스타트Built-In Hot Start
반응 수1000 Reactions
오버행Blunt
중합효소AccuPrime Pfx DNA Polymerase
수량1,000 reactions
반응 형식Separate Components
배송 조건Wet or Dry Ice
크기(최종 제품)12 kb or less
부피500 μL
검출 방법Primer-probe
용도(애플리케이션)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceLow
반응 속도Standard
Unit SizeEach
구성 및 보관
• AccuPrime Pfx DNA Polymerase (1 x 500 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (5 x 1 mL)
• 50 mM MgSO4 (2 x 1 mL)

Store at -20°C.