293fectin™ Transfection Reagent
293fectin™ Transfection Reagent
인용 및 참조 문헌 (3)
Gibco™

293fectin™ Transfection Reagent

293fectin™ Transfection Reagent은 무혈청 GIBCO™ FreeStyle™ 293 Expression Medium에서 FreeStyle™ 293-F 부유세포의 이입 효율성을 최대화하도록 특별히 만들어진 양이온 지질 formulation입니다자세히 알아보기
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카탈로그 번호수량
123470191 mL
12347500
12347-500으로도 사용됨
15 mL
12347750
12347-750으로도 사용됨
10 x 15 mL
카탈로그 번호 12347019
제품 가격(KRW)
852,000
Each
카트에 추가하기
수량:
1 mL
제품 가격(KRW)
852,000
Each
카트에 추가하기
293fectin™ Transfection Reagent은 무혈청 GIBCO™ FreeStyle™ 293 Expression Medium에서 FreeStyle™ 293-F 부유세포의 이입 효율성을 최대화하도록 특별히 만들어진 양이온 지질 formulation입니다 . 이는 FreeStyle™ 293 Expression System의 일부나 별도로 구매가능합니다.

이 시스템에 대한 자세한 정보는 FreeStyle™ 293 Expression System Brochure에서 찾아볼 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인293fectin
제품 유형Transfection Reagent
수량1 mL
혈청 호환 가능Yes
셀 유형Established Cell Lines
샘플 종류Plasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Each unit contains one vial of reagent. Store at 4°C.
293fectin™ is guaranteed stable for 6 months when stored properly.

자주 묻는 질문(FAQ)

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all 293fectin™ Transfection Reagent, 1 mL FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.
Authors:Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K,
Journal:J Biol Chem
PubMed ID:14701821
'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought ... More
Binding of anthrax toxin to its receptor is similar to alpha integrin-ligand interactions.
Authors:Bradley KA, Mogridge J, Jonah G, Rainey A, Batty S, Young JA,
Journal:J Biol Chem
PubMed ID:14507921
The secreted protein toxin produced by Bacillus anthracis contributes to virulence of this pathogen and can cause many of the symptoms seen during an anthrax infection, including shock and sudden death. The cell-binding component of anthrax toxin, protective antigen, mediates entry of the toxin into cells by first binding directly ... More
T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface.
Authors:Cao E, Zang X, Ramagopal UA, Mukhopadhaya A, Fedorov A, Fedorov E, Zencheck WD, Lary JW, Cole JL, Deng H, Xiao H, Dilorenzo TP, Allison JP, Nathenson SG, Almo SC,
Journal:Immunity
PubMed ID:17363302
The T cell immunoglobulin mucin (Tim) family of receptors regulates effector CD4(+) T cell functions and is implicated in autoimmune and allergic diseases. Tim-3 induces immunological tolerance, and engagement of the Tim-3 immunoglobulin variable (IgV) domain by galectin-9 is important for appropriate termination of T helper 1-immune responses. The 2 ... More