Medium 199, Hanks' Balanced Salts
Medium 199, Hanks' Balanced Salts
인용 및 참조 문헌 (1)
Gibco™

Medium 199, Hanks' Balanced Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation자세히 알아보기
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카탈로그 번호수량
12350039500 mL
카탈로그 번호 12350039
제품 가격(KRW)
77,000
キャンペーン価格
Ends: 31-Dec-2025
81,000
할인액 4,000 (5%)
Each
카트에 추가하기
수량:
500 mL
Customize this product
제품 가격(KRW)
77,000
キャンペーン価格
Ends: 31-Dec-2025
81,000
할인액 4,000 (5%)
Each
카트에 추가하기
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Medium 199 is modified as follows:
With
• Hanks' salts
• L-glutamine
• HEPES
• Phenol Red

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO2.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ Medium 199 product made in our Scotland facility (22350-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Medium 199 contains no proteins, lipids, or growth factors. Therefore, Medium 199 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO2 environment to maintain physiological pH.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주Rat epithelial
세포 유형Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형Medium 199
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin
형태Liquid
멸균Sterile-filtered
첨가제 포함Low Glucose, Glutamine, HEPES, Phenol Red
첨가제 없음No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medium 199, Hanks' Balanced Salts FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
TNF-related activation-induced cytokine (TRANCE) induces angiogenesis through the activation of Src and phospholipase C (PLC) in human endothelial cells.
Authors: Kim Young-Mi; Kim Young-Myoung; Lee You Mie; Kim Hae-Sun; Kim Jong Dai; Choi Yongwon; Kim Kyu-Won; Lee Soo-Young; Kwon Young-Guen;
Journal:J Biol Chem
PubMed ID:11741951
Angiogenesis is an essential step for many physiological and pathological processes. Tumor necrosis factor (TNF) superfamily cytokines are increasingly recognized as key modulators of angiogenesis. In this study, we tested whether TNF-related activation-induced cytokine (TRANCE), a new member of the TNF superfamily, possesses angiogenic activity in vitro and in vivo. ... More