Platinum™ SuperFi™ DNA Polymerase
해당 제품을 구매한 고객들이 함께 검색한 제품을 추천해드립니다. 온라인 할인 진행 중!
Platinum™ SuperFi™ DNA Polymerase
Invitrogen™

Platinum™ SuperFi™ DNA Polymerase

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호반응 수
12351010100 Reactions
12351050500 Reactions
123512505 x 500 Reactions
카탈로그 번호 12351010
제품 가격(KRW)
340,000
Each
카트에 추가하기
반응 수:
100 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
340,000
Each
카트에 추가하기
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Benefits of Platinum SuperFi DNA Polymerase

  • Exceptional >300X Taq fidelity
  • High specificity and increased yields with Platinum hot-start technology
  • Robust amplification of difficult targets including those of sub-optimal purity or high GC content
  • Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
  • Amplification of samples with suboptimal purity

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)300X
핫 스타트Built-In Hot Start
반응 수100 Reactions
오버행Blunt
중합효소Platinum SuperFi DNA Polymerase
수량100 units
반응 형식Separate Components
배송 조건Wet or Dry Ice
크기(최종 제품)20 kb or less
용도(애플리케이션)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도Fast
Unit SizeEach
구성 및 보관
• Platinum SuperFi DNA Polymerase (50 μL at 2 U/μL)
• 5X SuperFi Buffer (2 x 1.25 mL)
• 5X SuperFi GC Enhancer (1.25 mL)

Store at –5°C to –30°C.

자주 묻는 질문(FAQ)

Can I use Taq polymerase to generate my gene of interest for directional TOPO cloning?

No, your gene of interest must be amplified with a proofreading polymerase such as Platinum SuperFi DNA Polymerase or AccuPrime Pfx DNA Polymerase that leaves blunt ends for directional TOPO cloning.

Which nucleotide analogues can be used with Platinum SuperFi DNA Polymerase?

Platinum SuperFi DNA Polymerase cannot read dUTP-derivatives or dITP in DNA templates, so the use of these analogues is not recommended.Platinum SuperFi DNA Polymerase can incorporate 7-deaza-dGTP and radiolabeled dNTPs. 

I am having difficulties amplifying long targets using Platinum SuperFi DNA Polymerase. What are your recommendations?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh and intact) and fresh primer solutions. Optimization steps to consider include decreasing denaturation and extension temperatures, lengthening extension times as recommended in the manual, and increasing template amounts.

Can Platinum SuperFi DNA Polymerase amplify AT-rich targets?

Yes. To improve amplification of AT-rich targets, we recommend reducing the extension temperature to 68 degrees C or add 5-15 mM Tetramethylammonium Chloride (TMAC).

Can Platinum SuperFi DNA Polymerase amplify GC-rich targets?

All Platinum SuperFi product formats are supplied with 5X SuperFi GC Enhancer which is optimized to improve amplification of GC-rich targets (recommended for use with targets containing >65% GC content)