Platinum™ SuperFi™ PCR Master Mix
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Platinum™ SuperFi™ PCR Master Mix
Invitrogen™

Platinum™ SuperFi™ PCR Master Mix

Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
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카탈로그 번호반응 수
12358010100 Reactions
12358050500 Reactions
123582505 x 500 Reactions
카탈로그 번호 12358010
제품 가격(KRW)
389,000
Online offer
Ends: 31-Dec-2025
457,000
할인액 68,000 (15%)
Each
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반응 수:
100 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
389,000
Online offer
Ends: 31-Dec-2025
457,000
할인액 68,000 (15%)
Each
카트에 추가하기
Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.

Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology. Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Features

  • Exceptional >300X Taq fidelity
  • High specificity and increased yields with Platinum hot-start technology
  • Robust amplification of difficult targets of high GC content
  • Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions
  • Amplification of samples with suboptimal purity

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Long PCR

Notes

  • Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases.
  • Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)300X
핫 스타트Built-In Hot Start
반응 수100 Reactions
오버행Blunt
중합효소Platinum SuperFi DNA Polymerase
수량100 reactions
반응 형식SuperMix or Master Mix
배송 조건Wet or Dry Ice
크기(최종 제품)20 kb or less
용도(애플리케이션)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도Fast
Unit SizeEach
구성 및 보관
• 2X Platinum SuperFi PCR Master Mix (2 x 1.25 mL)
• SuperFi GC Enhancer (1.25 mL)
• Nuclease-free water (2 x 1.25 mL)

Store at –5°C to –30°C.

자주 묻는 질문(FAQ)

Which nucleotide analogues can be used with Platinum SuperFi DNA Polymerase?

Platinum SuperFi DNA Polymerase cannot read dUTP-derivatives or dITP in DNA templates, so the use of these analogues is not recommended.Platinum SuperFi DNA Polymerase can incorporate 7-deaza-dGTP and radiolabeled dNTPs. 

I am having difficulties amplifying long targets using Platinum SuperFi DNA Polymerase. What are your recommendations?

Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh and intact) and fresh primer solutions. Optimization steps to consider include decreasing denaturation and extension temperatures, lengthening extension times as recommended in the manual, and increasing template amounts.

Can Platinum SuperFi DNA Polymerase amplify AT-rich targets?

Yes. To improve amplification of AT-rich targets, we recommend reducing the extension temperature to 68 degrees C or add 5-15 mM Tetramethylammonium Chloride (TMAC).

Can Platinum SuperFi DNA Polymerase amplify GC-rich targets?

All Platinum SuperFi product formats are supplied with 5X SuperFi GC Enhancer which is optimized to improve amplification of GC-rich targets (recommended for use with targets containing >65% GC content)

Does SuperFi Green Buffer influence performance of Platinum SuperFi DNA Polymerase?

No. The colored buffer does not interfere with PCR performance and does not change enzyme features.