Search
인용 및 참조 문헌 (2)
Gibco™
Ham's F-10 Nutrient Mix, HEPES
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 12390035 | 500 mL |
카탈로그 번호 12390035
제품 가격(KRW)
77,000
Online offer
Ends: 31-Mar-2026
85,000할인액 8,000 (9%)
Each
수량:
500 mL
제품 가격(KRW)
77,000
Online offer
Ends: 31-Mar-2026
85,000할인액 8,000 (9%)
Each
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
The complete formulation is available.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ F-10 product made in our Scotland facility (22390-017). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This product is not used for in vitro diagnostic purpose in some countries.
This F10 is modified as follows:
| With |
| • Phenol Red |
| • HEPES |
| • L-glutamine |
The complete formulation is available.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ F-10 product made in our Scotland facility (22390-017). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This product is not used for in vitro diagnostic purpose in some countries.
사양
세포주CHO, COS-7, and rat prostate epithelial cells
세포 유형Primary Rat Astrocytes
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형Ham's F-10 Nutrient Mixture
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
How can I remove mycoplasma contamination from my cell culture medium?
I see a decrease in growth of my culture. What should I do?
My cells are not adhering to the culture vessel. What should I do?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
The carboxyl terminus of GLUT4 contains a serine-leucine-leucine sequence that functions as a potent internalization motif in Chinese hamster ovary cells.
Journal:J Biol Chem
PubMed ID:8702815
'To characterize the trafficking motifs contained in the carboxyl terminus of GLUT4, a chimera (GTCTR) was constructed in which the carboxyl-terminal 30 amino acids of GLUT4 were substituted for the amino-terminal cytoplasmic domain of the transferrin receptor (TR). The endocytic behavior of this chimera was characterized in Chinese hamster ovary
Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11504951
'Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term