IMDM
IMDM
인용 및 참조 문헌 (1)
Gibco™

IMDM

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.자세히 알아보기
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카탈로그 번호수량
1244006110 x 500 mL
12440053500 mL
124400461000 mL
124400796 x 1000 mL
카탈로그 번호 12440061
제품 가격(KRW)
545,000
線上優惠
Ends: 31-Dec-2025
573,000
할인액 28,000 (5%)
Each
카트에 추가하기
수량:
10 x 500 mL
Customize this product
제품 가격(KRW)
545,000
線上優惠
Ends: 31-Dec-2025
573,000
할인액 28,000 (5%)
Each
카트에 추가하기
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This IMDM is modified as follows:
With Without
• L-glutamine • α-thioglycerol
• Phenol Red • 2-mercaptoethanol

The complete formulation is available.

Using IMDM
IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
IMDM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical IMDM product made in our Scotland facility (21980-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주Jurkat, COS-7, and macrophage cells
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형IMDM (Iscove's Modified Dulbecco's Medium)
수량10 x 500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
첨가제 없음No α-Thioglycerol, No 2-Mercaptoethanol
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

Is IMDM (Cat. No. 12440061) also available without folic acid or folate?

Unfortunately, we do not offer any supplements without folic acid or folate. We would recommend going to this page (https://www.thermofisher.com/us/en/home/global/forms/biopro-info-gibco-bioprocessing.html) and filling out the form to request a quote for a custom IMDM, if feasible, made to your specifications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all IMDM, 10 x 500 mL FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Ex vivo differentiation of cord blood stem cells into megakaryocytes and platelets.
Authors:Pineault N, Robert A, Cortin V, Boyer L
Journal:Methods Mol Biol
PubMed ID:23179834
Megakaryocytes (MK) are hematopoietic cells present in the bone marrow that are responsible for the production and release of platelets in the circulation. Given their very low frequency (<1%), human MK often need to be derived in culture to study their development or to generate sufficient material for biological studies. ... More