Platinum™ PCR SuperMix High Fidelity

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인용 및 참조 문헌 (3)

Invitrogen™

Platinum™ PCR SuperMix High Fidelity

Name: Platinum™ PCR SuperMix High Fidelity
SKU: 12532016
Price: 486000 KRW

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

보기 방식 변경

카탈로그 번호	반응 수
12532016	100 Reactions
12532024	5000 Reactions

2 개 옵션

카탈로그 번호 12532016

제품 가격(KRW)

486,000

Each

카트에 추가하기

반응 수:

100 Reactions

대량 주문 또는 맞춤형 요청

제품 가격(KRW)

486,000

Each

카트에 추가하기

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.

High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.

Benefits of Platinum PCR SuperMix, High Fidelity

Six-times higher fidelity than Taq DNA polymerase
Amplifies fragments up to 15 kb
Minimizes PCR optimization
Room-temperature reaction assembly

Applications

High-fidelity PCR
Cloning
Mutagenesis

Notes

Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

For Research Use Only. Not for use in diagnostic procedures.

사양

Fidelity (Taq 대비)6X

핫 스타트Built-In Hot Start

반응 수100 Reactions

오버행Mixed

중합효소Platinum Taq DNA Polymerase High Fidelity

수량100 rxns

반응 형식SuperMix or Master Mix

배송 조건Wet or Dry Ice

크기(최종 제품)15 kb or less

농도1.1X

용도(애플리케이션)Hot-start PCR, High-fidelity PCR

GC-Rich PCR PerformanceLow

반응 속도Standard

Unit SizeEach

구성 및 보관

Platinum PCR SuperMix High Fidelity (4 x 1.125 mL)

Store at -20°C in a non-frost-free freezer.

자주 묻는 질문(FAQ)

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use MgCl2 instead of MgSO4 with Platinum Taq High Fidelity enzyme?

We strongly suggest using MgSO4. While MgCl2 may work in some cases, MgSO4 usually produces more robust and reproducible products, as sulfate is the best anion found for the Platinum Taq High Fidelity enzyme.

If I do PCR using the Platinum PCR SuperMix High Fidelity, can I use DNA-RNA chimeric oligos such as 5'-AGGCTTggau (lower case letters for RNA bases)?

PCR cannot be performed with an RNA oligo. RNA is not heat stable so the RNA part of the oligo will be degraded. In the presence of Mg++ (PCR buffer contains usually 1-2 mM Mg++) the rate of degradation is much faster.

인용 및 참조 문헌 (3)

인용 및 참조 문헌

Abstract

Identifying genes of agronomic importance in maize by screening microsatellites for evidence of selection during domestication.

Authors: Vigouroux Y; McMullen M; Hittinger C T; Houchins K; Schulz L; Kresovich S; Matsuoka Y; Doebley J;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12105270

'Crop species experienced strong selective pressure directed at genes controlling traits of agronomic importance during their domestication and subsequent episodes of selective breeding. Consequently, these genes are expected to exhibit the signature of selection. We screened 501 maize genes for the signature of selection using microsatellites or simple sequence repeats ... More

Pattern of diversity in the genomic region near the maize domestication gene tb1.

Authors:Clark RM, Linton E, Messing J, Doebley JF,

Journal:Proc Natl Acad Sci U S A

PubMed ID:14701910

Domesticated maize and its wild ancestor (teosinte) differ strikingly in morphology and afford an opportunity to examine the connection between strong selection and diversity in a major crop species. The tb1 gene largely controls the increase in apical dominance in maize relative to teosinte, and a region of the tb1 ... More

Transforming growth factor-ß is required for vasculogenic mimicry formation in glioma cell line U251MG.

Authors:Ling G, Wang S, Song Z, Sun X, Liu Y, Jiang X, Cai Y, Du M, Ke Y,

Journal:Cancer Biol Ther

PubMed ID:22104964

Both vasculogenic mimicry (VM) and transforming growth factor-ß (TGFß) are positively correlated with malignancy in glioma. Accordingly, we supposed that TGFß might be related with VM, and aimed to detect whether TGFß could influence VM formation in two glioma cell lines U251MG and SHG44, which were different in malignancy. We ... More