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인용 및 참조 문헌 (2)
Invitrogen™
PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells
Gateway™ Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 12535029 | 20 Reactions |
카탈로그 번호 12535029
제품 가격(KRW)
-
수량:
20 Reactions
Gateway™ Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway™ Technology. The PCR Cloning System with Gateway™ Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway™ Technology offers:
• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™
In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™
In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
For Research Use Only. Not for use in diagnostic procedures.
사양
클로닝 방법Gateway™
반응 수20 reactions
제품라인One Shot
제품 유형PCR Cloning System
수량20 Reactions
벡터pDONR
Unit SizeEach
구성 및 보관
The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reagents, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.
자주 묻는 질문(FAQ)
Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?
Do you have a recommended single-step protocol for BP/LR recombination?
How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?
Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?
Do you offer Gateway vectors for expression in plants?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
Participation of an Endomembrane Cation/H+ Exchanger AtCHX20 in Osmoregulation of Guard Cells.
Journal:Plant Physiol
PubMed ID:17337534
'Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized, however less is known about transport at endomembranes. CHX20, a member of a
Cytosolic Hydroxymethyldihydropterin Pyrophosphokinase/Dihydropteroate Synthase from Arabidopsis thaliana: A SPECIFIC ROLE IN EARLY DEVELOPMENT AND STRESS RESPONSE.
Journal:J Biol Chem
PubMed ID:17289662
In plants, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase (mitHPPK/DHPS) is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. Mining the Arabidopsis genome data base has revealed a second gene encoding a protein that lacks a potential transit peptide, suggesting a cytosolic localization of the isoenzyme (cytHPPK/DHPS). When