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MEM α, nucleosides
인용 및 참조 문헌 (4)
Gibco™

MEM α, nucleosides

MEM α (Minimum Essential Medium α)는 포유류 세포 배양뿐만 아니라 transfected DHFR-negative cell의 선별에도 널리 사용됩니다.
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카탈로그 번호수량
1257107110 x 500 mL
125710631 x 500 mL
125710481 x 1000 mL
125710896 x 1000 mL
카탈로그 번호 12571071
제품 가격(KRW)
630,000
Online offer
Ends: 30-Sep-2026
700,000
할인액 70,000 (10%)
Each
수량:
10 x 500 mL
Customize this product
제품 가격(KRW)
630,000
Online offer
Ends: 30-Sep-2026
700,000
할인액 70,000 (10%)
Each

MEM α (Minimum Essential Medium α)는 포유류 세포 배양뿐만 아니라 transfected DHFR-negative cell의 선별에도 널리 사용됩니다. MEM α는 keratinocyte, primary rat astrocyte 및 human melanoma cell을 포함한 다양한 부유 및 부착 포유류 세포의 배양에 사용할 수 있습니다. 다양한 세포 배양 응용 분야에 사용할 수 있도록 여러 조성의 MEM α 제품을 제공합니다. Media Selector Tool을 사용하여 용도에 적합한 조성을 확인해보세요.

본 MEM α의 조성은 다음과 같습니다.

포함: Ribonucleosides, Deoxyribonucleosides, Phenol Red, L-glutamine

전체 조성 정보를 확인할 수 있습니다.

MEM α 사용

MEM α는 Minimum Essential Medium (MEM)을 변형한 배지로, non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin 및 ascorbic acid를 함유하고 있습니다. Nucleoside가 포함되지 않은 MEM α도 제공되며, DG44 및 기타 DHFR-negative cell의 선별 배지로 사용할 수 있습니다. 본 제품은 Earle's salts를 사용하여 제조됩니다. MEM α에는 protein, lipid 또는 growth factor가 포함되어 있지 않습니다. 따라서 일반적으로 10% Fetal Bovine Serum (FBS)을 첨가하여 사용해야 합니다. MEM α는 sodium bicarbonate buffer system (2.2 g/L)을 사용하므로 생리학적 pH를 유지하려면 5–10% CO2 환경이 필요합니다.

연구용 또는 추가 제조용으로 사용하십시오. 진단용 또는 사람이나 동물에 직접 투여하는 용도로 사용할 수 없습니다.

사양
세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
세포 유형Mammalian Cells
농도1X
배양 환경CO2
용도(애플리케이션)Mammalian Cell Culture
형식Bottles
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형MEM α (Minimum Essential Medium α)
수량10 x 500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Ambient
분류Animal Origin-free
Culture TypeMammalian Cell Culture
형태Liquid
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
첨가제 없음No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

What is the difference between MEM α, nucleosides and MEM α, no nucleosides?

Our MEM α, nucleosides is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Our MEM α without nucleosides can be used a selection medium for DG44 and other DHFR-negative cells (DHFR = dihydrofolate reductase).
DHFR-negative cells are unable to grow unless the culture medium is supplemented with essential metabolites, including hypoxanthine and thymidine.

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM α, nucleosides FAQs

인용 및 참조 문헌 (4)

인용 및 참조 문헌
Abstract
Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
Authors:Cheng Z, Ito S, Nishio N, Xiao H, Zhang R, Suzuki H, Okawa Y, Murohara T, Isobe K
Journal:J Mol Cell Biol
PubMed ID:21228011
'If induced pluripotent stem (iPS) cells are to be used to treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPS cells from their tissues. To determine the feasibility of using this technology with elderly patients, we asked if it was indeed possible to ... More
A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics.
Authors:Shi S, Condon RG, Deng L, Saunders J, Hung F, Tsao YS, Liu Z
Journal:J Vis Exp
PubMed ID:21968840
'The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated ... More
Specific inhibition of ICAM-1 expression mediated by gene targeting with Triplex-forming oligonucleotides.
Authors:Besch R, Giovannangeli C, Kammerbauer C, Degitz K
Journal:J Biol Chem
PubMed ID:12080053
Selected sequences in the DNA double helix can be specifically recognized by oligonucleotides via hydrogen bonding interactions. The resulting triple helix can modulate DNA metabolism and especially interfere with transcription in a gene-specific manner. To explore the potential of triplex-forming oligonucleotides (TFOs) as gene repressors, a TFO was designed to ... More
Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites. Characterization of a novel role for the oligosaccharides in the in vitro and in vivo bioactivity.
Authors: Grossmann M; Szkudlinski M W; Tropea J E; Bishop L A; Thotakura N R; Schofield P R; Weintraub B D;
Journal:J Biol Chem
PubMed ID:7493973
We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and mutant hTSH in cell lines with different glycosylation patterns. The in vitro ... More