Mammalian Lumio™ Gateway™ Vectors, with Lumio™ Green In-Cell Detection Kit

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,자세히 알아보기

카탈로그 번호	수량
12589016	1 kit

카탈로그 번호 12589016

제품 가격(KRW)

1 kit

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

검출 위치In-Cell Detection

용도(애플리케이션)Reporter Assays

제품 유형Vector with In-cell Detection Kit

수량1 kit

선택 제제(진핵)Blasticidin

벡터pcDNA

클로닝 방법Gateway

제품라인Gateway, Lumio

프로모터CMV

단백질 태그Lumio, V5 Epitope Tag

Unit SizeEach

구성 및 보관

All destination vectors are provided lyophilized and supercoiled.

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Mammalian Lumio™ Gateway™ Vectors, with Lumio™ Green In-Cell Detection Kit FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

Identification of a lipase-linked cell-membrane receptor for pigment epithelium-derived factor (PEDF).

Authors:Notari L, Baladron V, Aroca-Aguilar JD, Balko N, Heredia R, Meyer C, Notario PM, Saravanamuthu S, Nueda ML, Sanchez-Sanchez F, Escribano J, Laborda J, Becerra SP,

Journal:J Biol Chem

PubMed ID:17032652

'PEDF is an extracellular multifunctional protein belonging to the serpin superfamily with demonstrable neurotrophic, gliastatic, neuronotrophic, antiangiogenic and antitumorigenic properties. We have previously provided biochemical evidence for high affinity PEDF-binding sites and proteins in plasma membranes of retina, retinoblastoma and CNS cells. This study was designed to reveal a receptor ... More