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Invitrogen™
SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
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보기 방식 변경
| 카탈로그 번호 | 포함 | 반응 수 |
|---|---|---|
| 12594100 | Kit only | 100 Reactions |
| 12595100 | Kit with ezDNase | 100 Reactions |
카탈로그 번호 12594100
제품 가격(KRW)
1,304,000
Online offer
Ends: 31-Dec-2025
1,448,000할인액 144,000 (10%)
Each
포함:
Kit only
반응 수:
100 Reactions
제품 가격(KRW)
1,304,000
Online offer
Ends: 31-Dec-2025
1,448,000할인액 144,000 (10%)
Each
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.
Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)
SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.
The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.
For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형PCR Amplified cDNA
형식Kit
핫 스타트Built-In Hot Start
포함Kit only
반응 수100 Reactions
최적 반응 온도45°C to 60°C
중합효소Platinum SuperFi
수량Each
반응 형식Master Mix
시약 유형Reverse Transcription
역전사 효소SuperScript IV
리보뉴클레아제 H 활성Reduced
배송 조건Dry Ice
크기(최종 제품)Up to 14 kb
시작 물질RNA
기술1-step RT-PCR
검출 방법Gel Electrophoresis
GC-Rich PCR PerformanceHigh
PCR 방법1-step RT-PCR
반응 속도10 min.
Unit SizeEach
구성 및 보관
• SuperScript IV RT Mix (50 μL)
• 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)
• Nuclease-free water (2 x 1.25 mL)
Store at –20°C.