TrypLE™ Express Enzyme (1X), no phenol red
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TrypLE™ Express Enzyme (1X), no phenol red
인용 및 참조 문헌 (4)
Gibco™

TrypLE™ Express Enzyme (1X), no phenol red

Green features
TrypLE™ Express는 동물 유래 성분이 없는 재조합 효소로 CHO, HEK 293, A529, 일차성 인간 각질화세포(primary human keratinocytes), 배아줄기세포(embryonic stem cell)자세히 알아보기
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카탈로그 번호수량
1260403920 x 100mL
12604013100 mL
12604021500 mL
126040545 L
카탈로그 번호 12604039
제품 가격(KRW)
606,000
Online offer
Ends: 31-Dec-2025
637,000
할인액 31,000 (5%)
Each
카트에 추가하기
수량:
20 x 100mL
Customize this product
제품 가격(KRW)
606,000
Online offer
Ends: 31-Dec-2025
637,000
할인액 31,000 (5%)
Each
카트에 추가하기
TrypLE™ Express는 동물 유래 성분이 없는 재조합 효소로 CHO, HEK 293, A529, 일차성 인간 각질화세포(primary human keratinocytes), 배아줄기세포(embryonic stem cell) 등 다양한 adherent 포유류 세포 해리에 사용합니다. 라이프 테크놀로지스는 다양한 세포 배양 어플리케이션을 위해 여러 가지 TrypLE™ 제형을 제공합니다. trypsin 및 다른 해리 시약과 비교한 Gibco™ TrypLE™ Express의 특징:

  • Gentle on cells - cell health를 유지하며 재현성 높은 결과를 제시합니다.
  • 실온 안정성 - 사용이 편리합니다.
  • 사용 용이성 - 비활성할 필요없이 기존 프로토콜에 바로 이용할 수 있습니다.
  • 동물 유래 성분 무함유(AOF) - 순수하고 일관적이며 효과적입니다.

TrypLE™ Express modification:

함유 무함유
• EDTA • Phenol Red


전체 제형은 기밀입니다. 자세한 정보는 Technical Services에 문의하세요.

Gentle on cells
TrypLE™ Express은 trypsin 처럼 lysine과 arginine의 C-terminal side 펩티드 결합을 절단합니다. 하지만 단일 효소 작용으로 인한 TrypLE™ Express‘ 의 우수한 순도로 특이성을 높입니다. 이로 trypsin 및 기타 추출물에서 여러 효소 절단으로 야기되는 손상을 줄입니다.
실온 안정성
TrypLE™ Express는 실온에서 안정적이며 즉시 사용이 가능합니다. TrypLE™ Express cell dissociation reagent은 실온 또는 2-8°C에서 24개월 동안 안정성을 유지해 보관과 취급이 쉽고 편리합니다.
사용 용이성
TrypLE™ Express는 기존 프로토콜에서 trypsin을 대체할 수 있습니다. 또한 희석 자체가 TrypLE™ Express을 비활성화시켜 FBS 같은 trypsin 억제제가 필요하지 않습니다.
동물 유래 성분 무함유(AOF)
돼지 trypsin과 기타 효소와 달리 TrypLE™ Express은 동물 유래 성분이 없습니다.
용도
이 제품은 연구용으로만 사용 가능합니다. 동물이나 인간에 진단 또는 치료용으로 사용할 수 없습니다.
cGMP 제조 품질 시스템
Gibco™ TrypLE™ Express는 뉴욕 Grand Island에 위치한 cGMP 부합 시설에서 제조됩니다. 이 시설은 의료기기 제조자로 FDA에 등록되어 있으며 ISO 13485, ISO 9001 인증을 받은 기관입니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells
킬레이터EDTA
농도1 X
배양 유형Adherent Cell Culture
그린 기능Energy efficient, Sustainable packaging
삼투압Check and Record
제품라인Gibco
수량20 x 100mL
비고Room Temperature Stable
유통 기한24 Months
배송 조건Room Temperature
소스Animal origin-free
테스트됨In vitro Bioassay
분류Animal Origin-free
형태Liquid
제품 유형Cell Culture Dissociation Reagent
멸균Sterile-filtered
첨가제 없음No Phenol Red
pH6 to 8
Unit SizeEach
구성 및 보관
Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

자주 묻는 질문(FAQ)

What enzymes do you recommend for passaging mesenchymal stem cells (MSC)?

We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?

Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell lines can I culture in Diploid Growth Serum-Reduced Medium (SRM)?

Diploid Growth Serum-Reduced Medium has been evaluated with fibroblast cell lines, including MRC-5, WI-38, IMR-90, BS-2, and CEF cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all TrypLE™ Express Enzyme (1X), no phenol red, 20 x 100mL FAQs

인용 및 참조 문헌 (4)

인용 및 참조 문헌
Abstract
Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells.
Authors:Aasen T, Izpisúa Belmonte JC,
Journal:Nat Protoc
PubMed ID:20134422
'The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing and infecting these cells. In ... More
VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin.
Authors:Ulyanova T, Scott LM, Priestley GV, Jiang Y, Nakamoto B, Koni PA, Papayannopoulou T,
Journal:Blood
PubMed ID:15769895
Although expression of vascular cell adhesion molecule 1 (VCAM-1) in endothelial cells and its functional implications have been previously appreciated, VCAM-1 expression in other than endothelial cells, especially hematopoietic cells, has been recently recognized and has not been explored in detail. Using normal mice and mice with a conditional ablation ... More
A rapid, cost-effective method for counting human embryonic stem cell numbers as clumps.
Authors:Prowse A, Wolvetang E, Gray P,
Journal:Biotechniques
PubMed ID:19594444
Enumeration of human embryonic stem cell (hESC) numbers through single cell digestion can be time consuming especially in high-throughput or multi-factorial analysis containing 50+ samples. We have developed a reproducible, cost-effective method of counting hESCs in clumps circumventing the need to manually dissociate each sample to single cells. The method ... More
Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.
Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,
Journal:Tissue Eng Part C Methods
PubMed ID:30412046
cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More