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인용 및 참조 문헌 (5)
Gibco™
Advanced RPMI 1640 Medium
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells자세히 알아보기
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| 카탈로그 번호 | 수량 |
|---|---|
| 12633012 | 500 mL |
| 12633020 | 10 x 500 mL |
카탈로그 번호 12633012
제품 가격(KRW)
65,000
Online offer
Ends: 30-Sep-2026
72,000할인액 7,000 (10%)
Each
수량:
500 mL
제품 가격(KRW)
65,000
Online offer
Ends: 30-Sep-2026
72,000할인액 7,000 (10%)
Each
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.
This Advanced RPMI 1640 is manufactured as follows:
With: glucose, non-essential amino acids, sodium pyruvate, phenol red
Without: L-glutamine, HEPES
The complete formulation is available.
Gibco Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX supplement. Many cell lines do not require adaptation to this medium. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주Sp2, Vero, Raji, Daudi, and Jurkat
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin
형태Liquid
Serum LevelReduced Serum
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
첨가제 없음No Glutamine, No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
What is the glucose concentration in Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020)?
Do you offer Advanced RPMI 1640 Medium (Cat. No. 12633012 or 12633020) without phenol red?
What is the difference between Advanced RPMI 1640 Medium and other RPMI 1640 formulations?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
인용 및 참조 문헌 (5)
인용 및 참조 문헌
Abstract
Viral complementation allows HIV-1 replication without integration.
Journal:Retrovirology
PubMed ID:18613957
BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA
Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids.
Journal:Nature communications
PubMed ID:26493500
Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute tissue-specific phenotypes is currently unknown. Here we show that hPSC-KCs self-organize into kidney organoids that functionally recapitulate tissue-specific epithelial physiology, including disease phenotypes after genome editing. In three-dimensional cultures, epiblast-stage hPSCs form spheroids
Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.
Journal:Scientific reports
PubMed ID:28831060
Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 × 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which
The G-Protein-Coupled Estrogen Receptor Agonist G-1 Inhibits Proliferation and Causes Apoptosis in Leukemia Cell Lines of T Lineage.
Journal:Frontiers in cell and developmental biology
PubMed ID:35237599
The G-protein–coupled estrogen receptor (GPER) mediates non-genomic action of estrogen. Due to its differential expression in some tumors as compared to the original healthy tissues, the GPER has been proposed as a therapeutic target. Accordingly, the non-steroidal GPER agonist G-1, which has often demonstrated marked cytotoxicity in experimental models, has
Nephron organoids derived from human pluripotent stem cells model kidney development and injury.
Journal:Nature biotechnology
PubMed ID:26458176
Kidney cells and tissues derived from human pluripotent stem cells (hPSCs) would enable organ regeneration, disease modeling, and drug screening in vitro. We established an efficient, chemically defined protocol for differentiating hPSCs into multipotent nephron progenitor cells (NPCs) that can form nephron-like structures. By recapitulating metanephric kidney development in vitro,