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인용 및 참조 문헌 (9)
Gibco™
Advanced DMEM/F-12
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells자세히 알아보기
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| 카탈로그 번호 | 수량 |
|---|---|
| 12634010 | 500 mL |
| 12634028 | 10 x 500 mL |
카탈로그 번호 12634010
제품 가격(KRW)
72,000
Online offer
Ends: 30-Sep-2026
79,000할인액 7,000 (9%)
Each
수량:
500 mL
제품 가격(KRW)
72,000
Online offer
Ends: 30-Sep-2026
79,000할인액 7,000 (9%)
Each
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows:
With: Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red
Without: L-glutamine, HEPES
The complete formulation is available.
Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주MRC-5, SP2, Vero, WI-38 and Jurkat
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM (Dulbecco's Modified Eagle Medium)/F-12
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin
형태Liquid
Serum LevelReduced Serum
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
첨가제 없음No Glutamine, No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
Can NSCs generated using PSC Neural Induction Medium be expanded in the PSC Neural Induction Medium or do they need to be in the recommended NSC expansion medium, as stated in the product manual?
Do you offer DMEM/F12 medium without folic acid?
What is the difference between Advanced DMEM/F12 Medium and DMEM/F12 Medium?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
인용 및 참조 문헌 (9)
인용 및 참조 문헌
Abstract
Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling.
Journal:Stem cells translational medicine
PubMed ID:28191779
Stem cell technologies, especially patient‐specific, induced stem cell pluripotency and directed differentiation, hold great promise for changing the landscape of medical therapies. Proper exploitation of these methods may lead to personalized organ transplants, but to regenerate organs, it is necessary to develop methods for assembling differentiated cells into functional, organ‐level
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Journal:Nature
PubMed ID:19329995
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while
In vitro generation of human pluripotent stem cell derived lung organoids.
Journal:eLife
PubMed ID:25803487
Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.
Journal:Nature
PubMed ID:23354049
The Wnt target gene Lgr5 marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon(1), stomach(2) and hair follicles(3). A 3D culture system allows long-term clonal expansion of single Lgr5(+) stem cells into transplantable organoids that retain many characteristics of the original epithelial architecture(2, 4,
Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics.
Journal:Nature communications
PubMed ID:30061675
Esophageal adenocarcinoma (EAC) incidence is increasing while 5-year survival rates remain less than 15%. A lack of experimental models has hampered progress. We have generated clinically annotated EAC organoid cultures that recapitulate the morphology, genomic, and transcriptomic landscape of the primary tumor including point mutations, copy number alterations, and mutational