Fetal Bovine Serum, mesenchymal stem cell-qualified, Australia origin
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Gibco™

Fetal Bovine Serum, mesenchymal stem cell-qualified, Australia origin

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Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of자세히 알아보기
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카탈로그 번호 12664025
제품 가격(KRW)
2,230,000
500 mL
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제품 가격(KRW)
2,230,000
500 mL
카트에 추가하기
Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Sera Category: Specialty
Specially tested for the ability to support the expansion and clonal enumeration (colony forming unit fibroblast assay) of mesenchymal stem cells. Endotoxin level: ≤10 EU⁄ml. Hemoglobin level: ≤25 mg⁄dl. Country of Origin: Australia
사양
AgeFetal
종Cattle/Bovine
원산지Australia
형태Liquid
형식Bottle
제품 유형Fetal Bovine Serum
수량500 mL
Serum TreatmentStandard (Sterile-filtered)
멸균Sterile
테스트됨Bovine Respiratory Syncytial Virus FA, Cytopathic Agents, Hemadsorbing Agents, Reovirus FA, Rabies FA, Hemoglobin (ng⁄dl), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Endotoxin (EU⁄ml), Mycoplasma, Sterility Testing (Bacteria & Fungi)
공여자 무리No
엔도톡신 농도≤ 10 EU/ml
그린 기능Sustainable packaging
헤모글로빈 농도≤ 25 mg⁄dl
순도 또는 품질 등급MS Cell-Qualified
제품라인Gibco™
배송 조건Dry Ice
Unit Size500 mL

자주 묻는 질문(FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sometimes I notice flocculence in serum. What could it be?

Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, one of the clot-forming proteins present in serum, after the serum has been thawed. This should not affect product performance. To remove the flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g. Then filter the resulting supernant along with your media. Do not attempt to filter serum containing flocculence, it may clog filters.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does material precipitate in FBS upon storage in the refrigerator?

Gibco FBS is not pre-aged. When stored at 2 to 8 degrees C, the possibility exists for various proteins and lipoproteins in serum (e.g., cold agglutinins, fibrinogen, vitronectin, etc.) to aggregate, and form either perceptible material or observed turbidity. This should not affect serum performance. We recommend that you store FBS at -20 degrees C and avoid repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.