Gibco™ Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf21 cells are adapted to serum-free suspension culture in Gibco™ Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco™ Sf21 cells (frozen in Gibco™ Sf-900™ III SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac™ Baculovirus Expression System, or the InsectDirect™ Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 10⁷ cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco™ Sf-900™ III SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Quality and performance testing
Each lot of Gibco™ Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.