BLOCK-iT™ Fluorescent Oligo, for electroporation

BLOCK-iT™ Fluorescent Oligo provides:• A strong, easily detectable FITC signal indicating transfection efficiency• Chemical modifications for a clearer, more persistent자세히 알아보기

카탈로그 번호	수량
13750062	75 μL

카탈로그 번호 13750062

제품 가격(KRW)

1,512,000

카트에 추가하기

75 μL

제품 가격(KRW)

1,512,000

카트에 추가하기

BLOCK-iT™ Fluorescent Oligo provides:

• A strong, easily detectable FITC signal indicating transfection efficiency
• Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA
• Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

This makes the BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. BLOCK-iT™ Fluorescent Oligo is available in two configurations: one designed for lipid-mediated transfection (2 x 125 μL) at 20 μM and one designed for electro-poration (75 μl) at 1 mM.

For Research Use Only. Not for use in diagnostic procedures.

제어 유형Transfection Control

검출 방법Fluorescence

용도(애플리케이션)Electroporation

라벨 또는 염료FITC (Fluorescein)

제품라인BLOCK-iT

제품 유형Fluorescent Oligo

수량75 μL

RNAi 유형siRNA

샘플 종류RNA

Unit SizeEach

구성 및 보관

The BLOCK-iT™ Fluorescent Oligo for electroporation is supplied as 75 μl at 1 mM, and should be stored at -20° C.

자주 묻는 질문(FAQ)

What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

We offer several negative and positive controls for Stealth siRNA:

For Stealth Positive Control:
- Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
- Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
- Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
- Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

For Stealth Negative Control:
Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

- Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
- Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
- Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
- Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
- Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
- For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.