HBSS (10X), calcium, magnesium, no phenol red

인용 및 참조 문헌 (1)

Gibco™

HBSS (10X), calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,자세히 알아보기

카탈로그 번호	수량
14065056	500 mL

카탈로그 번호 14065056

제품 가격(KRW)

79,000

Online offer

Ends: 31-Dec-2025

83,000

할인액 4,000 (5%)

카트에 추가하기

500 mL

Customize this product

제품 가격(KRW)

79,000

Online offer

Ends: 31-Dec-2025

83,000

할인액 4,000 (5%)

카트에 추가하기

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation. Concentrated forms of Gibco™ HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.

This HBSS is modified as follows:

With	Without
• Calcium	• Phenol Red
• Magnesium	• Sodium Bicarbonate
• Glucose

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco™ HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ HBSS product made in our Scotland facility (14065-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

사양

화학물질 이름 또는 재질Hanks' Balanced Salt Solution (HBSS)

포함No Sodium Pyruvate

제조 품질cGMP-compliant under the ISO 13485 standard

삼투압2700 - 3070 mOsm/kg

권장 스토리지Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

멸균Sterile-filtered

농도10 X

용도(애플리케이션)Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting And Preparing Reagents

물리적 형태Liquid

제품라인Gibco

수량500 mL

용액 유형Hank's Balanced Salt

pH4.4 - 4.7

Unit SizeEach

자주 묻는 질문(FAQ)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

The morphological and molecular features of the epithelial-to-mesenchymal transition.

Authors:Moreno-Bueno G, Peinado H, Molina P, Olmeda D, Cubillo E, Santos V, Palacios J, Portillo F, Cano A,

Journal:Nat Protoc

PubMed ID:19834475

Here we describe several methods for the characterization of epithelial-mesenchymal transition (EMT) at the cellular, molecular and behavioral level. This protocol describes both in vitro and in vivo approaches designed to analyze different features that when taken together permit the characterization of cells undergoing transient or stable EMT. We define ... More