Versene Solution
Versene Solution
인용 및 참조 문헌 (2)
Gibco™

Versene Solution

Versene Solution은 부드러운 비효소적 세포 분리 시약으로 사용되는 EDTA 용액입니다. Gibco™ Versene Solution (0.48 mM)은 인산완충식염수(PBS) 1리터당 0.2 g의 EDTA(Na4)로자세히 알아보기
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카탈로그 번호수량
15040066100 mL
카탈로그 번호 15040066
제품 가격(KRW)
30,000
온라인 행사
Ends: 31-Dec-2025
31,000
할인액 1,000 (3%)
Each
카트에 추가하기
수량:
100 mL
제품 가격(KRW)
30,000
온라인 행사
Ends: 31-Dec-2025
31,000
할인액 1,000 (3%)
Each
카트에 추가하기

Versene Solution은 부드러운 비효소적 세포 분리 시약으로 사용되는 EDTA 용액입니다. Gibco™ Versene Solution (0.48 mM)은 인산완충식염수(PBS) 1리터당 0.2 g의 EDTA(Na4)로 조성되어 있습니다.

사용 목적 및 안내
연구용으로만 사용 가능합니다. 진단 절차에는 사용할 수 없습니다.

cGMP 제조 및 품질 시스템
안정적인 공급을 위해, Gibco™ Versene Solution은 미국 뉴욕 Grand Island, 영국 스코틀랜드 두 곳의 시설에서 제조됩니다. 두 시설 모두 cGMP 제조 기준을 준수하며, ISO 13485 인증을 보유하고 있습니다.

For Research Use Only. Not for use in diagnostic procedures.

사양
킬레이터EDTA
제조 품질cGMP for medical devices, 21 CFR Part 820 and ISO 13485
수량100 mL
유통 기한18 Months
배송 조건Room Temperature
분류Animal Origin-free
제품 유형Cell Culture Dissociation Reagent
멸균Sterile-filtered
첨가제 포함EDTA
Unit SizeEach
구성 및 보관
Storage conditions: 2 to 8°C
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

자주 묻는 질문(FAQ)

I currently use mTeSR1. How difficult would it be to adapt my cells to the StemFlex Medium system?

This transition is very straightforward. We recommend at least a 2-passage transition into the StemFlex Medium system. Briefly, if you have cryopreserved cells previously cultured in the mTeSR1/Matrigel system, we recommend thawing the cells back into mTeSR1/Matrigel until fully recovered. Upon reaching ~70-85% confluency, passage using Versene solution and seed directly into the StemFlex Medium system.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Can I use EDTA or versene for passaging of stem cells?

Yes, you may use EDTA or versene. However, we do not recommend using dispase or collagenase as it can lead to differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add a ROCK inhibitor during passaging when culturing in Gibco Essential 8 Flex Medium?

As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is Gibco Versene Solution still the recommended dissociation reagent with Gibco Essential 8 Flex Medium?

Both Versene Solution (Cat. No. 15040066) and enzyme-mediated dissociation have been shown to be compatible passaging methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Decreased metallation and activity in subsets of mutant superoxide dismutases associated with familial amyotrophic lateral sclerosis.
Authors: Hayward Lawrence J; Rodriguez Jorge A; Kim Ji W; Tiwari Ashutosh; Goto Joy J; Cabelli Diane E; Valentine Joan Selverstone; Brown Robert H Jr;
Journal:J Biol Chem
PubMed ID:11854284
Over 90 different mutations in the gene encoding copper/zinc superoxide dismutase (SOD1) cause approximately 2% of amyotrophic lateral sclerosis (ALS) cases by an unknown mechanism. We engineered 14 different human ALS-related SOD1 mutants and obtained high yields of biologically metallated proteins from an Sf21 insect cell expression system. Both the ... More
Molecular determinants of receptor binding and signaling by the CX3C chemokine fractalkine.
Authors: Mizoue L S; Sullivan S K; King D S; Kledal T N; Schwartz T W; Bacon K B; Handel T M;
Journal:J Biol Chem
PubMed ID:11432858
Fractalkine/CX3CL1 is a membrane-tethered chemokine that functions as a chemoattractant and adhesion protein by interacting with the receptor CX3CR1. To understand the molecular basis for the interaction, an extensive mutagenesis study of fractalkine's chemokine domain was undertaken. The results reveal a cluster of basic residues (Lys-8, Lys-15, Lys-37, Arg-45, and ... More