인용 및 참조 문헌 (23)

Gibco™

Penicillin-Streptomycin (5,000 U/mL)

본 제품은 그램 양성균과 그램 음성균에 효과적인 활성을 보이는 penicillin와 streptomycin 항생제가 복합되어 있어 세포 배양 시 세균 오염을 예방하기자세히 알아보기

카탈로그 번호	수량
15070063	100 mL

카탈로그 번호 15070063

제품 가격(KRW)

43,000

キャンペーン価格

Ends: 31-Mar-2026

47,000

할인액 4,000 (9%)

100 mL

제품 가격(KRW)

43,000

キャンペーン価格

Ends: 31-Mar-2026

47,000

할인액 4,000 (9%)

본 제품은 그램 양성균과 그램 음성균에 효과적인 활성을 보이는 penicillin와 streptomycin 항생제가 복합되어 있어 세포 배양 시 세균 오염을 예방하기 위하여 사용할 수 있습니다. Penicillin은 원래 진균인 Penicillium에서 정제된 물질로 세균 세포벽 전환을 직접적으로 저해하고 세포벽을 변형시키는 효소 분비를 유도하는 간접 억제 작용을 합니다. Streptomycin는 원래 Streptomyces griseus에서 정제된 물질입니다. Streptomycin은 세균 리소좀 30S에 결합해 단백질 합성을 억제하고 감수성 높은 세균의 사멸을 유도합니다. 라이프 테크놀로지스는 세포 배양 어플리케이션에 사용하는 다양한 항생∙항진균제 를 제공합니다.

이 솔루션에는 penicillin 5000 단위와 streptomycin 5000 μg/ml가 함유되어 있습니다.

용도
이 제품은 연구용으로만 사용할 수 있습니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.

For Research Use Only. Not for use in diagnostic procedures.

농도100 X

배양 유형Mammalian Cell Culture, Insect Cell Culture

용도(애플리케이션)Prevention of Cell Culture Contamination

수량100 mL

유통 기한12 Months

배송 조건Dry Ice

형태Liquid

제품 유형Antibiotic

멸균Sterile-filtered

Unit SizeEach

구성 및 보관

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (23)

인용 및 참조 문헌

Abstract

Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.

Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; BÃƒÂ¶hm Michael; Nickenig Georg;

Journal:Circulation

PubMed ID:12021231

BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More

Directed differentiation of pancreatic δ cells from human pluripotent stem cells.

Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H

Journal:Nature communications

PubMed ID:39068220

Dysfunction of pancreatic Î´ cells contributes to the etiology of diabetes. Despite their important role, human Î´ cells are scarce, limiting physiological studies and drug discovery targeting Î´ cells. To date, no directed Î´-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.

Authors: Watters Jyoti J; Sommer Julie A; Pfeiffer Zachary A; Prabhu Usha; Guerra Alma N; Bertics Paul J;

Journal:J Biol Chem

PubMed ID:11786532

'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in ... More

Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

Authors: Zabzdyr Jennifer L; Lillard Sheri J;

Journal:Anal Chem

PubMed ID:11985318

'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More

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