Pierce™ Glutathione Coated Plates
Pierce™ Glutathione Coated Plates
Thermo Scientific™

Pierce™ Glutathione Coated Plates

Thermo Scientific™ Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.
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카탈로그 번호색상형식검출 방법
15240WhiteStandardChemiluminescence
15340BlackStandardFluorescence
15140ClearStrip PlateColorimetric
카탈로그 번호 15240
제품 가격(KRW)
448,000
온라인 행사
Ends: 31-Dec-2025
526,000
할인액 78,000 (15%)
Each
카트에 추가하기
색상:
White
형식:
Standard
검출 방법:
Chemiluminescence
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
448,000
온라인 행사
Ends: 31-Dec-2025
526,000
할인액 78,000 (15%)
Each
카트에 추가하기
Pierce Glutathione Coated Plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.
Thermo Scientific Pierce Glutathione Coated Plates are microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of Glutathione Coated Plates include:
Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with SuperBlock Blocking Buffer to reduce nonspecific binding

For Research Use Only. Not for use in diagnostic procedures.
사양
결합 특성Standard Capacity, Affinity
검출 방법Chemiluminescence
포장5 Plates
플레이트 블로킹SuperBlock™ Buffer
제품 유형Microplate
수량5 plates
색상White
형식Standard
물질Polystyrene
웰 수96
제품라인Pierce
표면 처리Glutathione
Unit SizeEach
구성 및 보관
Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag with desiccant at 4°C.

자주 묻는 질문(FAQ)

My initial primary culture is contaminated, what can I do?

Contamination of primary tissue could possibly be carried over to culture preparation. Change the media and treat with antibiotics to eliminate the contaminants. To prevent this in the future, wash tissue pieces several times in a balanced salt solution containing a higher concentration of antibiotics and antimycotics before starting culture.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the detection limit of proteins with Pierce Glutathione Coated Plates?

The background is extremely low when using the Pierce Glutathione Coated Plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).

How long are Pierce Glutathione Coated Plates stable?

The Pierce Glutathione Coated Plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.

Can I determine the concentration of an unknown GST-tagged protein?

To determine the approximate concentration of a GST-tagged protein, a standard curve should be generated using purified GST added in different concentrations to the wells of the plates. Please note that it is only the approximately concentration that can be determined since not 100% of the GST-fusion proteins will be bound due to denaturing or steric hindrance to GSH.

Can I whirl-mix solutions containing GST proteins?

No. Do not whirl-mix a solution containing GST-tagged proteins. The CV will increase due to slight degradation of the GST protein.