Trypan Blue Solution, 0.4%
Trypan Blue Solution, 0.4%
인용 및 참조 문헌 (3)
Gibco™

Trypan Blue Solution, 0.4%

막 여과, 0.85% 식염수에서 조제.자세히 알아보기
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카탈로그 번호수량
15250061100 mL
카탈로그 번호 15250061
제품 가격(KRW)
40,000
Online offer
Ends: 31-Dec-2025
42,000
할인액 2,000 (5%)
Each
카트에 추가하기
수량:
100 mL
제품 가격(KRW)
40,000
Online offer
Ends: 31-Dec-2025
42,000
할인액 2,000 (5%)
Each
카트에 추가하기
막 여과, 0.85% 식염수에서 조제.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells
색상Blue
농도0.004
검출 방법Colorimetric
용도(애플리케이션)Cell Counting
수량100 mL
유통 기한36 Months
배송 조건Room Temperature
형태Liquid
제품 유형Trypan Blue
멸균Sterile-filtered
Unit SizeEach
구성 및 보관
Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

자주 묻는 질문(FAQ)

How do I quench any fluorescence outside of my live cells and leave my intracellular staining intact?

There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Gibco Trypan Blue Solution, 0.4% (Cat. No. 15250061) sterilized?

Trypan Blue is filter sterilized through a 0.22 µm filter.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is Trypan Blue Solution, 0.4% dissolved in?

It is a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can cells be exposed to trypan blue before all cells are stained with the dye?

Unfortunately, there is no definitive answer to this question. The rate at which the cell-impermeant dye is absorbed depends on the cell type, their state of health, nourishment, engulfment activity, etc.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What causes trypan blue to form a precipitate?

Exposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Trypan Blue Solution, 0.4% FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Bax and Bak independently promote cytochrome C release from mitochondria.
Authors: Degenhardt Kurt; Sundararajan Ramya; Lindsten Tullia; Thompson Craig; White Eileen;
Journal:J Biol Chem
PubMed ID:11836241
'Pro-apoptotic Bax and Bak have been implicated in the regulation of p53-dependent apoptosis. We assessed the ability of primary baby mouse kidney (BMK) epithelial cells from bax(-/-), bak(-/-), and bax(-/-) bak(-/-) mice to be transformed by E1A alone or in conjunction with dominant-negative p53 (p53DD). Although E1A alone transformed BMK ... More
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Authors: Esumi Hiroyasu; Izuishi Kunihiko; Kato Kazuyoshi; Hashimoto Koichi; Kurashima Yukiko; Kishimoto Atsuhiro; Ogura Tsutomu; Ozawa Takayuki;
Journal:J Biol Chem
PubMed ID:12091379
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia; ... More