X-Gal
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X-Gal
Invitrogen™

X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
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카탈로그 번호수량제품 유형
15520034100 mgX-Gal Substrate
155200181 gX-Gal Substrate
카탈로그 번호 15520034
제품 가격(KRW)
156,000
Online offer
Ends: 31-Dec-2025
183,000
할인액 27,000 (15%)
Each
카트에 추가하기
수량:
100 mg
제품 유형:
X-Gal Substrate
제품 가격(KRW)
156,000
Online offer
Ends: 31-Dec-2025
183,000
할인액 27,000 (15%)
Each
카트에 추가하기
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.

Primary applications

  • Blue-white screening—detect functional β-galactosidase (reporter gene expression)
  • Histochemical detection of β-galactosidase activity in fixed tissues
  • X-gal overlay assay

Find the media format that fits your needs

We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034).

We also offer IPTG (15529019) for blue-white screening.

Explore other media formats for bacterial growth.

For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 이름 또는 재질5-bromo-4-chloro-3-indolyl-β-D-galactoside
설명X-Gal, Cell-Permeant
용도(애플리케이션)Immunocytochemical Assays
제품 유형X-Gal Substrate
수량100 mg
배송 조건Approved for shipment at Room Temperature or on Wet Ice
검출 방법Colorimetric
형식Bottle
Unit SizeEach
구성 및 보관
Store in freezer (-5°C to -30°C).

자주 묻는 질문(FAQ)

Can X-gal be resuspended in dimethylsulfoxide (DMSO) instead of dimethylformamide (DMF)? How are X-gal plates prepared for blue/white screening?

X-gal (also known as 5-bromo-4-chloro-3-indolyl-?-D-galactoside) is soluble in either DMSO or DMF, and can be stored in solution in the freezer for up to 6 months. Protect the solution from light.

To add X-gal to previously made agar plates, pipette 40 ?l of a 40 mg/ml stock solution onto a 100mm pre-poured agar plate and spread evenly with a sterile spreader. Let dry 15 minutes and store plates away from light.

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.