100 bp DNA Ladder
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100 bp DNA Ladder
인용 및 참조 문헌 (1)
Invitrogen™

100 bp DNA Ladder

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Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100자세히 알아보기
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카탈로그 번호수량
15628019100 Applications
15628050500 Applications
카탈로그 번호 15628019
제품 가격(KRW)
168,000
Online offer
Ends: 31-Dec-2025
197,000
할인액 29,000 (15%)
Each
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수량:
100 Applications
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
168,000
Online offer
Ends: 31-Dec-2025
197,000
할인액 29,000 (15%)
Each
카트에 추가하기
Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.

100 bp DNA Ladder is ideal for separation on 1–2% agarose gels.

Highlights of 100 bp DNA Ladder:
• Sharp, clear bands—chromatography purified fragments for consistent and reliable results
• Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration
• Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 1–2% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.

For Research Use Only. Not for use in diagnostic procedures.
사양
농도0.5 μg/μL
젤 호환성Agarose gel
그린 기능Sustainable packaging
키트 구성품100 μL DNA Ladder, 1 mL 10X Sample Loading Buffer, 100 Applications
반응 수100 Applications
제품 유형DNA Ladder
수량100 Applications
로드 준비No
샘플 로딩 부피1 mL
배송 조건Approved for shipment at Room Temperature or on Dry Ice
기술Individual chromatography-purified DNA fragments
부피(미터법)100 μL
젤 유형Agarose
크기 범위100 to 2000 bp
Unit SizeEach
구성 및 보관
• 100 µL 100 bp DNA Ladder
• 1 mL 10X BlueJuice Gel Loading Buffer

Store at -20°C.

자주 묻는 질문(FAQ)

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?

Invitrogen DNA ladders are composed of double-stranded DNA fragments only.

Why are the DNA bands from my molecular weight ladder smearing?

Here are a few reasons why you might see smearing of the bands:

- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.

I'm seeing anomalous migration of my DNA ladder. What happened?

This can happen if the marker was heated. Please ensure that the ladders are not heated before use.

View all 100 bp DNA Ladder, 100 μL DNA Ladder, 1 mL 10X Sample Loading Buffer, 100 Applications FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Prostaglandin E2 transactivates EGF receptor: a novel mechanism for promoting colon cancer growth and gastrointestinal hypertrophy.
Authors: Pai Rama; Soreghan Brian; Szabo Imre L; Pavelka Meredith; Baatar Dolgor; Tarnawski Andrzej S;
Journal:Nat Med
PubMed ID:11875501
'Prostaglandins (PGs), bioactive lipid molecules produced by cyclooxygenase enzymes (COX-1 and COX-2), have diverse biological activities, including growth-promoting actions on gastrointestinal mucosa. They are also implicated in the growth of colonic polyps and cancers. However, the precise mechanisms of these trophic actions of PGs remain unclear. As activation of the ... More