Gentamicin (50 mg/mL)
Gentamicin (50 mg/mL)
인용 및 참조 문헌 (5)
Gibco™

Gentamicin (50 mg/mL)

Gentamicin sulfate는 진균 Micromonospora purpurea에서 정제한 수용성 항생제입니다. Gentamicin은 박테리아 리소좀 30S에 결합해 단백질 합성을 억제하고 감수성 높은 세균의 사멸을자세히 알아보기
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카탈로그 번호수량
1575007810 x 10 mL
1575006010 mL
카탈로그 번호 15750078
제품 가격(KRW)
912,000
Online offer
Ends: 31-Dec-2025
959,000
할인액 47,000 (5%)
Each
카트에 추가하기
수량:
10 x 10 mL
제품 가격(KRW)
912,000
Online offer
Ends: 31-Dec-2025
959,000
할인액 47,000 (5%)
Each
카트에 추가하기
Gentamicin sulfate는 진균 Micromonospora purpurea에서 정제한 수용성 항생제입니다. Gentamicin은 박테리아 리소좀 30S에 결합해 단백질 합성을 억제하고 감수성 높은 세균의 사멸을 유도합니다. Gibco™ Gentamicin은 다양한 그램 양성 및 그램 음성 박테리아에 효과적으로 세포 배양 시 박테리아 오염을 방지하기 위해 사용합니다. 농도 0.5∼ 50 μg ⁄ ml 범위에서 사용하기를 권장합니다. 라이프 테크놀로지스는 세포 배양 어플리케이션에 사용하는 다양한 항생∙항진균제 를 제공합니다.

용도
이 제품은 연구용으로만 사용해야 합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.

cGMP 두 곳에서 제조
Gibco™ Gentamicin은 뉴욕 Grand Island에 소재한 cGMP에 부합하는 시설에서 제조됩니다. 이 시설은 의료기기 제조자로 FDA에 등록되어 있으며 ISO 13485, ISO 9001 인증을 받은 기관입니다. 공급망 유지를 위해 라이프 테크놀로지스는 본사 스코틀랜드 시설(15710-049)에서도 동일한 Gibco™ Gentamicin 제품을 제공합니다. 이 시설은 의료기기 제조자로 FDA에 등록되어 있으며 ISO 13485 인증을 받은 기관입니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
농도50 mg/mL
권장 스토리지Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
배송 조건Room Temperature
멸균 필터링됨Yes
검증된 애플리케이션Prevention of Cell Culture Contamination
물리적 형태Liquid
수량10 x 10 mL
유형Gentamicin
Unit SizeEach

자주 묻는 질문(FAQ)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,
Journal:J Biol Chem
PubMed ID:22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
A three-dimensional co-culture system to investigate macrophage-dependent tumor cell invasion.
Authors:Dwyer AR, Ellies LG, Holme AL, Pixley FJ
Journal:J Biol Methods
PubMed ID:31453214
'Macrophages infiltrate cancers and promote progression to invasion and metastasis. To directly examine tumor-associated macrophages (TAMs) and tumor cells interacting and co-migrating in a three-dimensional (3D) environment, we have developed a co-culture model that uses a PyVmT mouse mammary tumor-derived cell line and mouse bone marrow-derived macrophages (BMM). The Py8119 ... More
Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic,
Authors:Nair DVT, Kollanoor Johny A
Journal:Front Microbiol
PubMed ID:30050507
'Antimicrobial potential of a dairy-origin probiotic bacteria,'
Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells.
Authors:Conroy LR, Lorkiewicz P, He L, Yin X, Zhang X, Rai SN, Clem BF
Journal:Cancer Cell Int
PubMed ID:32624705
Aberrant activity of cell cycle proteins is one of the key somatic events in non-small cell lung cancer (NSCLC) pathogenesis. In most NSCLC cases, the retinoblastoma protein tumor suppressor (RB) becomes inactivated via constitutive phosphorylation by cyclin dependent kinase (CDK) 4/6, leading to uncontrolled cell proliferation. Palbociclib, a small molecule ... More