Fetal Bovine Serum, 인증, 미국
Fetal Bovine Serum, 인증, 미국
인용 및 참조 문헌 (10)
Gibco™

Fetal Bovine Serum, 인증, 미국

Gibco fetal bovine sera는 기본 세포 배양, 특수 연구 및 특정 분석에 뛰어난 가치를 제공하며, 연구자의 연구 요구 사항과 예산자세히 알아보기
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카탈로그 번호수량형식
16000044500 mL병
Premium Plus Fetal Bovine Serum (FBS) 은 안정성 및 제조 공정은 그대로 유지되면서 품질이 더욱 강화되었습니다.
카탈로그 번호 16000044
현재 제품 선택
Gibco fetal bovine sera는 기본 세포 배양, 특수 연구 및 특정 분석에 뛰어난 가치를 제공하며, 연구자의 연구 요구 사항과 예산 요구 사항을 충족할 수 있도록 일관된 품질 및 우수성을 널리 인정받은 지원을 통해 신뢰할 수 있는 결과를 제공합니다.

Gibco 혈청: 일관된 성능을 위한 믿을 수 있는 품질

혈청 카테고리: Premium(Performance Plus)
•광범위한 세포 유형, 특히 민감한 세포주에 사용됩니다.
• 가장 낮은 엔도톡신 및 헤모글로빈 함량을 제공하는 당사의 가장 특성화된 혈청
• 엔도톡신 함량: ≤ 5 EU/mL
• 헤모글로빈 함량: ≤ 15 mg/dL
• 원산지: 미국

Gibco 혈청이 제공하는 이점
• ISO 13485 인증, FDA 등록 시설에서 처리
• 체외 진단용 라벨(최고 수준의 USP 멸균 시험 준수)
• 0.1 μm에서 삼중 필터링됨
• Gibco 병은 후드 안에서도 사용이 간편하고 오염 위험을 줄이며 세포 배양을 보다 일관성 있게 수행할 수 있도록 도와줍니다.
• 떼어내기 쉬운 라벨 스티커는 실험실 노트북에 붙여서 로트 번호 및 유효기간 기록을 쉽게 참조할 수 있도록 해줍니다.
이 제품은 체외 진단용입니다.
사양
엔도톡신 농도≤5 EU/mL
헤모글로빈 농도≤15 mg/dL
순도 또는 품질 등급인증
배송 조건냉동 상태
종소
Age태아
원산지미국
형태액체
형식병
제품 유형소 태아 혈청(Fetal Bovine Serum)
수량500 mL
Serum TreatmentStandard (Sterile-Filtered)
멸균멸균
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
구성 및 보관
보관 조건: ≤-10°C
배송 조건: 냉동 상태

자주 묻는 질문(FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, 인증, 미국 FAQs

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
Fibroblast growth factor receptor-1 signaling induces osteopontin expression and vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro.
Authors: Li Guohong; Oparil Suzanne; Kelpke Stacey S; Chen Yiu-Fai; Thompson John A;
Journal:Circulation
PubMed ID:12176960
'BACKGROUND: Increased expression of osteopontin (OPN), fibroblast growth factors (FGFs), and their type-1 receptor (FGFR-1) is associated with neointima formation and atherosclerosis. This study tested the hypothesis that ligand activation of FGFR-1 stimulates OPN expression in rat aortic smooth muscle cells (RASMCs), explored the signaling pathway involved, and assessed the ... More
Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.
Authors:Li T, Dai W, Lu L.
Journal:J Biol Chem
PubMed ID:12082101
'The exposure of mammalian cells to UV irradiation induces the expression of immediate early genes such as c-jun and c-fos and activates the transcription factors AP-1 and NF-kappaB. JunD is one of the three members of the Jun family and shares some functional characteristics with c-Jun. In the present study, ... More
Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.
Authors: Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;
Journal:Nat Med
PubMed ID:12118246
Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More
Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival.
Authors: Sotiriou Sotiria; Gispert Suzana; Cheng Jun; Wang Yaohui; Chen Amy; Hoogstraten-Miller Shelley; Miller Georgina F; Kwon Oran; Levine Mark; Guttentag Susan H; Nussbaum Robert L;
Journal:Nat Med
PubMed ID:11984580
The only proven requirement for ascorbic acid (vitamin C) is in preventing scurvy, presumably because it is a cofactor for hydroxylases required for post-translational modifications that stabilize collagen. We have created mice deficient in the mouse ortholog (solute carrier family 23 member 1 or Slc23a1) of a rat ascorbic-acid transporter, ... More
Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device.
Authors: Yang Mengsu; Li Cheuk-Wing; Yang Jun;
Journal:Anal Chem
PubMed ID:12199565
We have developed a microfluidic device for on-chip monitoring of cellular reactions. The device consists of two primary analytical functions: control of cell transport and immobilization, and dilution of an analyte solution to generate a concentration gradient. In this device, a dam structure in parallel to the fluid flow was ... More
View all Fetal Bovine Serum, 인증, 미국 citations