FreeStyle™ MAX Reagent
FreeStyle™ MAX Reagent
Gibco™

FreeStyle™ MAX Reagent

FreeStyle™ MAX System은 신속하고 높은 수율로 mammalian protein 생성을 할 수 있는 첨단 기술입니다. 이 새로운 mammalian expression 기술은 두자세히 알아보기
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카탈로그 번호수량
164471001 mL
1644750015 mL
1644775010 x 15 mL
카탈로그 번호 16447100
제품 가격(KRW)
977,000
온라인 행사
Ends: 31-Dec-2025
1,085,000
할인액 108,000 (10%)
Each
카트에 추가하기
수량:
1 mL
제품 가격(KRW)
977,000
온라인 행사
Ends: 31-Dec-2025
1,085,000
할인액 108,000 (10%)
Each
카트에 추가하기
FreeStyle™ MAX System은 신속하고 높은 수율로 mammalian protein 생성을 할 수 있는 첨단 기술입니다. 이 새로운 mammalian expression 기술은 두 가지 편리한 형식으로 이용할 수 있습니다. FreeStyle™ MAX Reagent는 새로운 cationic lipid-based reagent로 FreeStyle™ cell transfection efficiency를 높이도록 만들어졌습니다. FreeStyle™ MAX Expression System은 GIBCO™ FreeStyle™ Media, FreeStyle™ MAX Reagent, FreeStyle™ CHO-S cell (또는 FreeStyle™ 293 cell)로 구성됩니다. FreeStyle™ MAX System을 사용하는데 필요한 장치는 CO2 incubator와 platform shaker, shake flask 또는 spinner culture 뿐입니다.

FreeStyle™ MAX System 특징:
• suspension culture에서 CHO와 293 cells의 transient transfection을 위한 쉽고 빠른 프로토콜(그림 1)
• Functional proteins (mg 단위까지)과 모든 post-translational modification이 1주일 내에 완성됨
• FreeStyle™ serum-free medium으로 분비된 단백질 downstream purification 간편함
• production scale-up이 가능한 확실한 프로토콜

FreeStyle™ MAX System은 시간이 많이 걸리는 stable cell line generation 과정을 1주일 안에 large-scale functional protein production으로 대체할 수 있습니다
(그림 2).

구성 및 보관:
FreeStyle™ MAX Reagent, +4°C에서 보관.
For Research Use Only. Not for use in diagnostic procedures.
사양
분류Animal Origin-Free
용도(애플리케이션)Transfection
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인Freestyle
제품 유형Transfection Reagent
수량1 mL
혈청 호환 가능Yes
배송 조건Wet Ice
셀 유형Established Cell Lines
샘플 종류Plasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Each unit contains one vial of reagent. Store at +4°C. FreeStyle™ MAX is guaranteed stable for 6 months when stored properly.

자주 묻는 질문(FAQ)

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all FreeStyle™ MAX Reagent, 1 mL FAQs