FreeStyle™ MAX Reagent
FreeStyle™ MAX Reagent
Actual product may vary
인용 및 참조 문헌 (1)
Gibco™

FreeStyle™ MAX Reagent

FreeStyle™ MAX System은 신속하고 높은 수율로 mammalian protein 생성을 할 수 있는 첨단 기술입니다. 이 새로운 mammalian expression 기술은 두자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
1644775010 x 15 mL
164471001 mL
1644750015 mL
카탈로그 번호 16447750
제품 가격(KRW)
98,977,000
Online offer
Ends: 31-Dec-2025
116,443,000
할인액 17,466,000 (15%)
Each
카트에 추가하기
수량:
10 x 15 mL
제품 가격(KRW)
98,977,000
Online offer
Ends: 31-Dec-2025
116,443,000
할인액 17,466,000 (15%)
Each
카트에 추가하기
FreeStyle™ MAX System은 신속하고 높은 수율로 mammalian protein 생성을 할 수 있는 첨단 기술입니다. 이 새로운 mammalian expression 기술은 두 가지 편리한 형식으로 이용할 수 있습니다. FreeStyle™ MAX Reagent는 새로운 cationic lipid-based reagent로 FreeStyle™ cell transfection efficiency를 높이도록 만들어졌습니다. FreeStyle™ MAX Expression System은 GIBCO™ FreeStyle™ Media, FreeStyle™ MAX Reagent, FreeStyle™ CHO-S cell (또는 FreeStyle™ 293 cell)로 구성됩니다. FreeStyle™ MAX System을 사용하는데 필요한 장치는 CO2 incubator와 platform shaker, shake flask 또는 spinner culture 뿐입니다.

FreeStyle™ MAX System 특징:
• suspension culture에서 CHO와 293 cells의 transient transfection을 위한 쉽고 빠른 프로토콜(그림 1)
• Functional proteins (mg 단위까지)과 모든 post-translational modification이 1주일 내에 완성됨
• FreeStyle™ serum-free medium으로 분비된 단백질 downstream purification 간편함
• production scale-up이 가능한 확실한 프로토콜

FreeStyle™ MAX System은 시간이 많이 걸리는 stable cell line generation 과정을 1주일 안에 large-scale functional protein production으로 대체할 수 있습니다
(그림 2).

구성 및 보관:
FreeStyle™ MAX Reagent, +4°C에서 보관.
For Research Use Only. Not for use in diagnostic procedures.
사양
분류Animal Origin-Free
용도(애플리케이션)Protein Expression
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인Freestyle
제품 유형Transfection Reagent
수량10 x 15 mL
혈청 호환 가능Yes
배송 조건Wet Ice
셀 유형Established Cell Lines
형식Tube
샘플 종류Plasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Each kit contains 10 units. Each unit contains one vial of reagent. Store at +4°C. FreeStyle™ MAX is guaranteed stable for 6 months when stored properly.

자주 묻는 질문(FAQ)

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the recommended ratio of DNA and transfection reagent when transfecting Freestyle cells?

The optimal amount will vary, but we recommend starting with a 1:1 ratio of DNA and lipid. For example, 1.25 ug DNA and 1.25 ul of the reagent for one ml of suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How does FreeStyle MAX reagent compare to Lipofectamine 2000 and 293fectin reagents?

Lipofectamine 2000 is designed for adherent cells. FreeStyle and 293fectin reagents are designed for suspended Hek293 and CHO cells. For transfecting 293 suspension cells, FreeStyle MAX reagent yields transfection efficiency comparable to Lipofectamine 2000 and 293fectin reagents. However, Lipofectamine 2000 and 293fectin reagents do not work well for CHO suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What's the transfection efficiency for FreeStyle MAX reagent?

In general, the transfection efficiency for FreeStyle MAX reagent is 65% when used with suspension CHO and 293 cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all FreeStyle™ MAX Reagent, 10 x 15 mL FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells.
Authors:Liu C, Dalby B, Chen W, Kilzer JM, Chiou HC,
Journal:Mol Biotechnol
PubMed ID:18327552
'The efficient transfection of cloned genes into mammalian cells system plays a critical role in the production of large quantities of recombinant proteins (r-proteins). In order to establish a simple and scaleable transient protein production system, we have used a cationic lipid-based transfection reagent-FreeStyle MAX to study transient transfection in ... More