McCoy's 5A (Modified) Medium
McCoy's 5A (Modified) Medium
인용 및 참조 문헌 (1)
Gibco™

McCoy's 5A (Modified) Medium

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established자세히 알아보기
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카탈로그 번호수량
1660010810 x 500 mL
16600082500 mL
카탈로그 번호 16600108
제품 가격(KRW)
426,000
온라인 행사
Ends: 31-Mar-2026
473,000
할인액 47,000 (10%)
Each
카트에 추가하기
수량:
10 x 500 mL
Customize this product
제품 가격(KRW)
426,000
온라인 행사
Ends: 31-Mar-2026
473,000
할인액 47,000 (10%)
Each
카트에 추가하기
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Bacto-peptone
• Phenol Red

The complete formulation is available.

Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
McCoy's 5A (modified) Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical McCoy's 5A (modified) Medium product made in our Scotland facility (26600-080). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.
사양
세포주Rat fibroblasts
세포 유형Biopsy Tissues
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형McCoy's 5A Modified Medium
수량10 x 500 mL
유통 기한12 Months From Date of Manufacture
분류Animal Origin
형태Liquid
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, Glutamine, Phenol Red, Bacto-peptone
첨가제 없음No HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all McCoy's 5A (Modified) Medium, 10 x 500 mL FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Authors: Kachman Maureen T; Wang Haixing; Schwartz Donald R; Cho Kathleen R; Lubman David M;
Journal:Anal Chem
PubMed ID:11985308
A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric ... More