Keratinocyte SFM(serum-free medium)은 세포 feeder layer를 확보할 필요가 없으며 인간 케라틴 세포와 기타 유형의 상피 세포의 성장 및 유지에 최적화되어자세히 알아보기
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17005042
500 mL
카탈로그 번호 17005042
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수량:
500 mL
제품 가격(KRW)
189,000
Online offer
Ends: 31-Dec-2025
209,000
할인액 20,000 (10%)
Each
카트에 추가하기
Keratinocyte SFM(serum-free medium)은 세포 feeder layer를 확보할 필요가 없으며 인간 케라틴 세포와 기타 유형의 상피 세포의 성장 및 유지에 최적화되어 있습니다. 이 완전 배지는 피부 대체물, 체외(in vitro) 독성학, 유전자 치료제와 관련된 연구에서 human epidermal keratinocyte를 배양하는 데 사용할 수 있습니다.
Keratinocyte SFM은 사용 시에 human recombinant epidermal growth factor (rEGF) 및 bovine pituitary extract (BPE)으로 보충하는 완전 무혈청 배지입니다. 보충제(supplements), rEGF와 BPE는 모두 기본 배지와 함께 별도의 포장으로 제공됩니다.
Are the Keratinocyte SFM basal medium and supplement kit available separately?
The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.
Which serum-free media can be used to culture mouse keratinocytes?
The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.
Which serum-free media can be used to culture human keratinocytes?
There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.
What is the calcium concentration in Keratinocyte SFM (1X) (Cat. No. 17005042)?
Keratinocyte SFM (1X) (Cat. No. 17005042) includes Keratinocyte-SFM (Cat. No. 10724011) and Keratinocytes Supplements (Cat. No. 37000015), which contains Bovine Pituitary Extract (BPE) EGF, Human Recombinant. The complete media is prepared by adding the supplements to the base media. The Keratinocyte-SFM (Cat. No. 10724011) contains calcium chloride at 12.98 mg/L and D calcium pantothenate at 0.29 mg/L.
For how long can I store Keratinocyte-SFM at 4 degrees C?
The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C