Collagenase, Type II, powder
Collagenase, Type II, powder
인용 및 참조 문헌 (2)
Gibco™

Collagenase, Type II, powder

이 type II collagenase 용액은 Clostridium histolyticum에서 조제됩니다. 이는 세포 및 조직 분해에 사용합니다.Type II collagenase는 다음 조직의 세포 사용에자세히 알아보기
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카탈로그 번호수량
171010151 g
카탈로그 번호 17101015
제품 가격(KRW)
489,000
Online offer
Ends: 31-Mar-2026
543,000
할인액 54,000 (10%)
Each
카트에 추가하기
수량:
1 g
제품 가격(KRW)
489,000
Online offer
Ends: 31-Mar-2026
543,000
할인액 54,000 (10%)
Each
카트에 추가하기
이 type II collagenase 용액은 Clostridium histolyticum에서 조제됩니다. 이는 세포 및 조직 분해에 사용합니다.

Type II collagenase는 다음 조직의 세포 사용에 권장됩니다.

간
뼈
갑상선
심장
침샘

Collagenase는 Pro-X-Glyc-Pro 시퀀스에서 중성 아미노산(x)와 글리신 결합에 특이성을 갖는 단백질 분해효소입니다. 이 시퀀스는 콜라겐에 매우 많이 존재합니다. Collagenase는 결합 조직에 많이 있는 triplehelical native collagen fibrils를 분해하는 능력이 있는 고유한 단백질 분해효소입니다.

라이프테크놀로지스의 세포배양 제품군의 일부분
라이프테크놀로지스는 배지, 보충제, 염용액, 버퍼, 화학제, 해리 시약, 형질전환 시약 등 다양한 시약을 제공하여 세포 배양에서 여러분이 필요로 하는 부분을 모두 충족해 드립니다. 본사의 우수한 품질관리 수준과 기술 지원은 여러분께서 만족하실 수준입니다.

이 제품은 연구용으로만 사용가능합니다. 인간이나 동물에게 치료 또는 진단용으로 사용할 수 없습니다.

관련 링크:

세포 해리 시약 알아보기

전체 세포 배양 제품 보기
For Research Use Only. Not for use in diagnostic procedures.
사양
수량1 g
유통 기한24 Months
배송 조건Room Temperature
형태Lyophilized
제품 유형Collagenase
멸균Non-sterile
Unit SizeEach
구성 및 보관
Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

자주 묻는 질문(FAQ)

Can I reconstitute Collagenase, Type II, powder (Cat. No. 17101015) into DMEM or MEM alpha instead of HBSS with Ca and Mg?

Yes, Collagenase, Type II, powder can be reconstituted in DMEM or MEM alpha as long as the Mg and Ca concentrations in the medium are similar.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I make the 1000X stock (100 U/µL) solution from Collagenase powder?

1. Add 1 mL Hanks' Balanced Salt Solution (HBSS) with calcium and magnesium directly to 1 g vial of Collagenase. Vortex gently to ensure complete dissolution. Transfer to a clean tube.
2. Determine volume of HBSS (with calcium and magnesium) required to bring collagenase solution to 100 U/µL (1000X stock solution). The activity is lot- specific. Rinse vial with this volume of HBSS (with calcium and magnesium), and combine. Filter sterilize 1000X stock solution with a low protein binding filtration unit.
Example: Assuming the lot you have purchased has an activity of 265 U/mg, this lot will have 265000 Units per mL when you reconstitute collagenase into HBSS (with calcium and magnesium) at 1 g/mL. In order to dilute 265000 U/L to 100000 U/mL (= 100 U/µL), you need to dilute the 1 g/mL enzyme solution 2.65 fold.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts.
Authors:Sander V, Suñe G, Jopling C, Morera C, Izpisua Belmonte JC,
Journal:Nat Protoc
PubMed ID:23538883
'This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts and culture them for up to 4 weeks, thereby using them as an alternative to in vivo experiments. After collagenase digestion of the ventricle, cells are exposed to increasing calcium concentrations in order to obtain high-purity cardiomyocytes. The ... More
The PDZ binding motif of the Beta 1 adrenergic receptor modulates receptor trafficking and signaling in cardiac myocytes.
Authors: Xiang Yang; Devic Eric; Kobilka Brian;
Journal:J Biol Chem
PubMed ID:12097326
'beta(1) and beta(2) adrenergic receptors (AR) regulate the intrinsic contraction rate in neonatal mouse cardiac myocytes through distinct signaling pathways. It has been shown that stimulation of beta(1)ARs leads to a protein kinase A-dependent increase in contraction rate. In contrast, stimulation of beta(2)ARs has a biphasic effect on contraction rate, ... More