N-2 Supplement (100X)

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Both N-2 Supplement formulations can be used in the culture of neurons, but the concentrations of components in the formula for the Global Stem N-2 Supplement are higher than the Gibco N-2 Supplement.

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The N-2 Supplement (100X) solution can be filtered. We do not recommend filtering the medium once N-2 has been added at a final concentration of 1X.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The shelf life for N-2 Supplement (100X) is 18 months from the date of manufacture when stored at the recommended storage condition of -5 to -20 degrees C, protected from light. The expiration date should be listed on the product label as well as on the COA for the lot of the supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

As a general guideline, we recommend using Gibco B-27 Plus Supplement to culture neural stem cells, hippocampal, and other CNS neurons.

- For neuroblastomas, or post-mitotic neurons from both PNS and CNS, Gibco N-2 Supplement can be used.
- For primary glial cells (astrocytes) or tumor cell lines of astrocytic phenotype (astrocytes and gliomas), or oligodendrocytes, Gibco G-5 Supplement can be used.