Taq DNA Polymerase, native
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<i>Taq</i> DNA Polymerase, native
인용 및 참조 문헌 (20)
Invitrogen™

Taq DNA Polymerase, native

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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카탈로그 번호수량
18038018100 Units
18038042500 Units
180380673 x 500 Units
180382405000 Units
카탈로그 번호 18038018
제품 가격(KRW)
111,000
線上優惠
Ends: 31-Dec-2025
130,000
할인액 19,000 (15%)
Each
카트에 추가하기
수량:
100 Units
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
111,000
線上優惠
Ends: 31-Dec-2025
130,000
할인액 19,000 (15%)
Each
카트에 추가하기
Taq DNA Polymerase, native, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This native enzyme is purified from Thermus aquaticus YT1 and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
사양
엑소뉴클레아제 활성5' - 3'
Fidelity (Taq 대비)1X
형식Stand-alone enzyme
핫 스타트No
반응 수100 Reactions
오버행3'-A
중합효소Taq DNA Polymerase
제품 유형DNA Polymerase
수량100 Units
반응 형식Separate Components
배송 조건Wet or Dry Ice
크기(최종 제품)5 kb or less
시작 물질DNA
농도5 U/μL
용도(애플리케이션)Standard PCR
GC-Rich PCR PerformanceLow
PCR 방법qPCR, Standard PCR
반응 속도Standard
Unit SizeEach
구성 및 보관
• Taq DNA Polymerase (5 U/μL), 20 μL
• 10X PCR buffer (without magnesium), 1.25 mL
• 50 mM MgCl2, 1 mL

Store at -20°C in a non-frost-free freezer.
Stable for 6 months when stored properly.

자주 묻는 질문(FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Taq DNA Polymerase, native FAQs

인용 및 참조 문헌 (20)

인용 및 참조 문헌
Abstract
Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Authors: Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M;
Journal:J Biol Chem
PubMed ID:11773047
'We have determined previously that Sp3 encodes three distinct gene products as follows: a full-length protein (Sp3) that is an activator of transcription and two isoforms (M1 and M2) derived via internal translational initiation that function as transcriptional repressors. To identify amino acids and functions required for transcriptional repression, we ... More
Both DNA and histone fold sequences contribute to archaeal nucleosome stability.
Authors: Bailey Kathryn A; Marc Frederic; Sandman Kathleen; Reeve John N;
Journal:J Biol Chem
PubMed ID:11751933
'The roles and interdependence of DNA sequence and archaeal histone fold structure in determining archaeal nucleosome stability and positioning have been determined and quantitated. The presence of four tandem copies of TTTAAAGCCG in the polylinker region of pLITMUS28 resulted in a DNA molecule with increased affinity (DeltaDeltaG of approximately 700 ... More
Nuclear factor-kappa B directs carcinoembryonic antigen-related cellular adhesion molecule 1 receptor expression in Neisseria gonorrhoeae-infected epithelial cells.
Authors: Muenzner Petra; Billker Oliver; Meyer Thomas F; Naumann Michael;
Journal:J Biol Chem
PubMed ID:11751883
'The human-specific pathogen Neisseria gonorrhoeae expresses opacity-associated (Opa) protein adhesins that bind to various members of the carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) family. In this study, we have analyzed the mechanism underlying N. gonorrhoeae-induced CEACAM up-regulation in epithelial cells. Epithelial cells represent the first barrier for the microbial pathogen. ... More
Methylation-mediated silencing of TMS1/ASC is accompanied by histone hypoacetylation and CpG island-localized changes in chromatin architecture.
Authors: Stimson Krista M; Vertino Paula M;
Journal:J Biol Chem
PubMed ID:11733524
'Aberrant methylation of CpG-dense islands in the promoter regions of genes is an acquired epigenetic alteration associated with the silencing of tumor suppressor genes in human cancers. In a screen for endogenous targets of methylation-mediated gene silencing, we identified a novel CpG island-associated gene, TMS1, which is aberrantly methylated and ... More
Functional coadaptation between cytochrome c and cytochrome c oxidase within allopatric populations of a marine copepod.
Authors: Rawson Paul D; Burton Ronald S;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12271133
'Geographically isolated populations may accumulate alleles that function well on their own genetic backgrounds but poorly on the genetic backgrounds of other populations. Consequently, interpopulation hybridization may produce offspring of low fitness as a result of incompatibilities arising in allopatry. Genes participating in these epistatic incompatibility systems remain largely unknown. ... More
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