Taq DNA Polymerase, native
<i>Taq</i> DNA Polymerase, native
인용 및 참조 문헌 (3)
Invitrogen™

Taq DNA Polymerase, native

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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카탈로그 번호수량
180380673 x 500 Units
18038018100 Units
18038042500 Units
180382405000 Units
카탈로그 번호 18038067
제품 가격(KRW)
1,460,000
Each
카트에 추가하기
수량:
3 x 500 Units
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,460,000
Each
카트에 추가하기
Taq DNA Polymerase, native, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This native enzyme is purified from Thermus aquaticus YT1 and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
사양
엑소뉴클레아제 활성5' - 3'
Fidelity (Taq 대비)1X
형식Stand-alone enzyme
핫 스타트No
반응 수1500 Reactions
오버행3'-A
중합효소Taq DNA Polymerase
제품 유형DNA Polymerase
수량3 x 500 Units
반응 형식Separate Components
배송 조건Wet or Dry Ice
크기(최종 제품)5 kb or less
시작 물질DNA
농도5 U/μL
용도(애플리케이션)Standard PCR
GC-Rich PCR PerformanceLow
PCR 방법qPCR, Standard PCR
반응 속도Standard
Unit SizeEach
구성 및 보관
• Taq DNA Polymerase (5 U/μL), 300 μL
• 10X PCR buffer (without magnesium), 7.5 mL
• 50 mM MgCl2, 3 mL

Store at -20°C in a non-frost-free freezer.
Stable for 6 months when stored properly.

자주 묻는 질문(FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Taq DNA Polymerase, native FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
p38 alpha mitogen-activated protein kinase is activated by CD28- mediated signaling and is required for IL-4 production by human CD4+CD45RO+ T cells and Th2 effector cells.
Authors:Schafer PH, Wadsworth SA, Wang L, Siekierka JJ
Journal:J Immunol
PubMed ID:10358155
'T cell proliferation and cytokine production usually require stimulation via both the TCR/CD3 complex and the CD28 costimulatory receptor. Using purified human CD4+ peripheral blood T cells, we show that CD28 stimulation alone activates p38 alpha mitogen-activated protein kinase (p38 alpha). Cell proliferation induced by CD28 stimulation alone, a response ... More
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
Authors: Olson J H; Xiang X; Ziegert T; Kittelson A; Rawls A; Bieber A L; Chandler D E;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11562501
Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in ... More
Association of IL12B promoter polymorphism with severity of atopic and non-atopic asthma in children.
Authors: Morahan Grant; Huang Dexing; Wu Mark; Holt Barbara; White Gregory; Kendall Garth; Sly Peter; Holt Patrick;
Journal:Lancet
PubMed ID:12241719
Background Severe asthma is a frequent cause of hospital admission, especially among children. The main environmental triggers of airway inflammation in asthma are viruses and aeroallergens. These agents elicit reciprocal immune responses, characterised by production of T helper 1 and T helper 2 cytokines, respectively. There is no genetic explanation ... More