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인용 및 참조 문헌 (3)
Invitrogen™
Uracil DNA Glycosylase
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 18054015 | 100 units |
카탈로그 번호 18054015
제품 가격(KRW)
286,000
Online offer
Ends: 31-Dec-2025
317,000할인액 31,000 (10%)
Each
수량:
100 units
제품 가격(KRW)
286,000
Online offer
Ends: 31-Dec-2025
317,000할인액 31,000 (10%)
Each
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of
enzyme in 50 μl for 10 min. at 37°C (4).
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of
enzyme in 50 μl for 10 min. at 37°C (4).
For Research Use Only. Not for use in diagnostic procedures.
사양
중합효소DNA Polymerase
수량100 units
배송 조건Approved for shipment on Wet or Dry Ice
용도(애플리케이션)Standard PCR, Real Time PCR (qPCR)
형태Liquid
PCR 방법qPCR
Unit SizeEach
구성 및 보관
Store in freezer (-5° to -30°C).
자주 묻는 질문(FAQ)
What is UDG?
인용 및 참조 문헌 (3)
인용 및 참조 문헌
Abstract
A novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis.
Journal:Nat Genet
PubMed ID:10545944
Hereditary haemochromatosis (HH), which affects some 1 in 400 and has an estimated carrier frequency of 1 in 10 individuals of Northern European descent, results in multi-organ dysfunction caused by increased iron deposition, and is treatable if detected early. Using linkage-disequilibrium and full haplotype analysis, we have identified a 250-kilobase
Structural determinants for HIV-1 integrase inhibition by beta-diketo acids.
Journal:J Biol Chem
PubMed ID:11805103
Among all the HIV-1 integrase inhibitors, the beta-diketo acids (DKAs) represent a major lead in anti-HIV-1 integrase drug design. These derivatives inhibit the integration reaction in vitro with a strong specificity for the 3'-end joining step. They are also antiviral and inhibit integration in vivo. The aim of the present
Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates.
Journal:J Biol Chem
PubMed ID:11960995
The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG). MutY is a base excision repair (BER) glycosylase that removes misincorporated adenine residues from OG:A mispairs, as well as G:A and C:A mispairs. We