M-MLV Reverse Transcriptase Buffer
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M-MLV Reverse Transcriptase Buffer
Invitrogen™

M-MLV Reverse Transcriptase Buffer

M-MLV Reverse Transcritase Buffer는 5X Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KC1, 15 mM MgCl2]와 500 μl 0.1자세히 알아보기
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카탈로그 번호수량
180570181 mL
카탈로그 번호 18057018
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105,000
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Ends: 31-Dec-2025
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수량:
1 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
105,000
Online offer
Ends: 31-Dec-2025
123,000
할인액 18,000 (15%)
Each
카트에 추가하기
M-MLV Reverse Transcritase Buffer는 5X Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KC1, 15 mM MgCl2]와 500 μl 0.1 M DTT로 공급됩니다. 이는 M-MLV, SuperScript™, SuperScript™ II, and SuperScript™ III reverse transcriptases에 사용이 가능합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
포함

• M-MLV Reverse Transcriptase Buffer (1 mL)
• 0.1 M DTT (500 μL)

Store at –20°C.

농도5X
용도(애플리케이션)Reverse Transcription
수량1 mL
Unit SizeEach

자주 묻는 질문(FAQ)

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

What is the highest temperature that MMLV, SuperScript II, SuperScript III, or SuperScript IV RTs can be used?

The optimal temperature for for MMLV is 42 degrees C. The optimal temperature for SuperScript II RT is 42 degrees C, and can be used up to 50 degrees C. The optimal temperature for SuperScript III RT is 50 degrees C, and can be used up to 55 degrees C. For some qRT-PCR reactions where gene-specific primers are used, you can do the RT reaction at 60 degrees C. The optimal temperature for SuperScript IV RT is 50 degrees C, but can be used up to 65 degrees C.

Can I use elevated temperatures for reverse transcription with Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase?

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is not well-suited for elevated temperatures. Typically, M-MLV RT operates optimally at temperatures around 37 degrees C to 42 degrees C.

To perform reverse transcription at elevated temperatures (e.g., 50 degrees C or higher), consider using thermostable reverse transcriptases such as SuperScript IV or Maxima H Minus, which are engineered to perform efficiently at higher temperatures.