SuperScript™ II 역전사 효소
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SuperScript™ II 역전사 효소
Invitrogen™

SuperScript™ II 역전사 효소

Invitrogen SuperScript II Reverse Transcriptase는 야생형 MMLV RT에 비해 RNase H 활성 감소 및 열 안정성이 증가하도록 유전적으로 설계된 MMLV자세히 알아보기
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카탈로그 번호반응 수
18064071200 Reactions
1806402210 Reactions
1806401450 Reactions
카탈로그 번호 18064071
제품 가격(KRW)
1,741,000
Exklusiv online
Ends: 31-Dec-2025
1,934,000
할인액 193,000 (10%)
Each
카트에 추가하기
반응 수:
200 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,741,000
Exklusiv online
Ends: 31-Dec-2025
1,934,000
할인액 193,000 (10%)
Each
카트에 추가하기
Invitrogen SuperScript II Reverse Transcriptase는 야생형 MMLV RT에 비해 RNase H 활성 감소 및 열 안정성이 증가하도록 유전적으로 설계된 MMLV 역전사 효소(RT)입니다. 효소의 RNase H 영역 돌연변이는 제1가닥 cDNA 합성 중에 RNA 분해를 제거하여 전체 길이 cDNA의 수율을 높여줍니다.

SuperScript RT는 현재까지 50,000 건이 넘는 인용, 검토 및 문헌과 함께 가장 신뢰성이 높고 널리 사용되는 RT입니다.

참고: SuperScript RT 제품군의 최신 제품인 SuperScript IV Reverse Transcriptase는 최적의 순도 또는 무결성을 포함한 RNA 시료 내 향상된 열안정성, 가공도(processivity), 수율 및 성능을 제공합니다.

연구용으로만 사용하십시오. 진단용으로는 사용할 수 없습니다.
사양
최종 제품 유형First-Strand cDNA
형식독립형 효소
반응 수200 Reactions
최적 반응 온도42°C
수량4 x 10,000 units
반응 형식Separate components
시약 유형Reverse Transcription
역전사 효소SuperScript II
리보뉴클레아제 H 활성Reduced
배송 조건Wet Ice
크기(최종 제품)Up to 12.3 kb
시작 물질RNA
기술Reverse Transcription
농도200 U/μL
GC-Rich PCR Performance높음
반응 속도표준
Unit SizeEach
구성 및 보관

• SuperScript II Reverse Transcriptase, 4 x 50 μL (4 x 10,000 units at 200 U/μL)
• 5X First-strand buffer
• DTT (100 mM)

Store at –20°C.

자주 묻는 질문(FAQ)

How can I remove genomic DNA contamination from my sample prior to performing RT-PCR?

We recommend using ezDNase (Cat. No. 11766051). ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55 degrees C). These features make ezDNase Enzyme an excellent choice for genomic DNA removal prior to reverse transcription reactions.

How much RNA should be employed for first-strand cDNA synthesis?

The amount of RNA template for a cDNA synthesis is highly flexible and depends upon the amount of sample available and an individual's need. In general, 1 µg total RNA is used in a typical 20-µL RT reaction.

Find additional tips, troubleshooting help, and resources within ourReverse Transcription and RACE Support Center.

Should I treat the cDNA with RNase H prior to downstream processing?

RNase H treatment is not always necessary. Many PCR reactions work without it. However, for cDNA synthesized with RNase H-deficient reverse transcriptases (like SuperScript II, III, and IV), RNA/cDNA hybrids—especially GC-rich ones—may not denature well, reducing PCR sensitivity. RNase H treatment can help in such cases. Additionally, RNase H treatment is beneficial for cloning larger fragments.

What percentage of RNA is converted to cDNA when performing reverse transcription?

This depends highly on the quality of the sample. mRNA itself makes up 1-5% of total RNA. Depending on the primer and enzyme used, reverse transcription can covert >70% of that into cDNA.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

I'm setting up my RT reaction and am trying to decide whether I should use random primers, oligo(dT) primer, gene-specific primer, or oligo(dT)/random mix primers. What would you suggest?

Random primers are the best choice for degraded RNA, RNA with heavy secondary structure, non-polyadenylated RNA, or prokaryotic RNA. It is recommended only for two-step RT-PCR, and typically gives the highest yields, although the cDNA may not necessarily be full length. Oligo(dT) primers are good to use when trying to recover full-length cDNA from 2-step RT-PCR. The reaction is influenced by secondary structure and RNA quality. Gene specific primers should be used for very specific, mainly one-step RT-PCR reactions.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

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