SuperScript™ III First-Strand Synthesis SuperMix

인용 및 참조 문헌 (4)

Invitrogen™

SuperScript™ III First-Strand Synthesis SuperMix

SuperScript® III First-Strand Synthesis SuperMix는 편리한 high-throughput supermix format으로 높은 first-stand cDNA 수율을 제공합니다. 이는 2X Reaction Mix와 SuperScript® III자세히 알아보기

카탈로그 번호	수량
18080400	50 rxns

카탈로그 번호 18080400

제품 가격(KRW)

754,000

Ends: 31-Mar-2026

886,000

할인액 132,000 (15%)

카트에 추가하기

50 rxns

대량 주문 또는 맞춤형 요청

제품 가격(KRW)

754,000

Ends: 31-Mar-2026

886,000

할인액 132,000 (15%)

카트에 추가하기

SuperScript® III First-Strand Synthesis SuperMix는 편리한 high-throughput supermix format으로 높은 first-stand cDNA 수율을 제공합니다. 이는 2X Reaction Mix와 SuperScript® III Enzyme Mix 단 두 시험관을 사용해 간편하고 시간이 절약됩니다(그림 1). 수율과 민감도 개선을 위해 annealing buffer가 옵션으로 제공됩니다. 이 Enzyme Mix는 SuperScript® III Reverse Transcriptase를 포함하여 높은 cDNA 수율과 0.1 pg까지의 우수한 민감도(그림 2) 및 특이성을 보장하며 다양한 RT-PCR 어플리케이션에 사용할 수 있습니다.

For Research Use Only. Not for use in diagnostic procedures.

최종 제품 유형First-Strand cDNA

형식Kit

반응 수50 Reactions

최적 반응 온도50°C

프라이머Random Primers, Oligo dT Primers

수량50 rxns

반응 형식Master Mix

시약 유형Reverse Transcription

역전사 효소SuperScript III

배송 조건Dry Ice

시작 물질RNA

기술Reverse Transcription

용도(애플리케이션)Real Time PCR (qPCR)

GC-Rich PCR PerformanceHigh

반응 속도50 min.

Unit SizeEach

구성 및 보관

• 2X Reaction Mix, 500 μL
• SuperScript™ III Enzyme Mix, 100 μL
• Oligo(dT)₂₀, 50 μL total (50 μM)
• Random hexamers, 50 μL (50 ng/μL)
• Annealing buffer, 50 μL

Store at –20°C.

자주 묻는 질문(FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

How can I remove genomic DNA contamination from my sample prior to performing RT-PCR?

We recommend using ezDNase (Cat. No. 11766051). ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55 degrees C). These features make ezDNase Enzyme an excellent choice for genomic DNA removal prior to reverse transcription reactions.

How much RNA should be employed for first-strand cDNA synthesis?

The amount of RNA template for a cDNA synthesis is highly flexible and depends upon the amount of sample available and an individual's need. In general, 1 µg total RNA is used in a typical 20-µL RT reaction.

Find additional tips, troubleshooting help, and resources within ourReverse Transcription and RACE Support Center.

Should I treat the cDNA with RNase H prior to downstream processing?

RNase H treatment is not always necessary. Many PCR reactions work without it. However, for cDNA synthesized with RNase H-deficient reverse transcriptases (like SuperScript II, III, and IV), RNA/cDNA hybrids—especially GC-rich ones—may not denature well, reducing PCR sensitivity. RNase H treatment can help in such cases. Additionally, RNase H treatment is beneficial for cloning larger fragments.

What percentage of RNA is converted to cDNA when performing reverse transcription?

This depends highly on the quality of the sample. mRNA itself makes up 1-5% of total RNA. Depending on the primer and enzyme used, reverse transcription can covert >70% of that into cDNA.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

View all SuperScript™ III First-Strand Synthesis SuperMix FAQs

인용 및 참조 문헌 (4)

인용 및 참조 문헌

Abstract

TDAG51 is an ERK signaling target that opposes ERK-mediated HME16C mammary epithelial cell transformation.

Authors:Oberst MD, Beberman SJ, Zhao L, Yin JJ, Ward Y, Kelly K,

Journal:BMC Cancer

PubMed ID:18597688

'INTRODUCTION: Signaling downstream of Ras is mediated by three major pathways, Raf/ERK, phosphatidylinositol 3 kinase (PI3K), and Ral guanine nucleotide exchange factor (RalGEF). Ras signal transduction pathways play an important role in breast cancer progression, as evidenced by the frequent over-expression of the Ras-activating epidermal growth factor receptors EGFR and ... More

Elevated serum IL-10 levels in diffuse large B-cell lymphoma: a mechanism of aberrant JAK2 activation.

Authors:Gupta M, Han JJ, Stenson M, Maurer M, Wellik L, Hu G, Ziesmer S, Dogan A, Witzig TE,

Journal:Blood

PubMed ID:22323454

'Cytokines are deregulated in cancers and can contribute to tumor growth. In patients with diffuse large-cell lymphoma (DLBCL), we observed higher levels of JAK/STAT pathway-related serum cytokines (ie, IL-6, IL-10, epidermal growth factor, and IL-2) compared with controls. Of these, only IL-10 activated the JAK2 pathway in lymphoma cells in ... More

Interactive effects of neurohypophyseal neuropeptides with receptor antagonists on passive avoidance behavior: mediation by a cerebral neurohypophyseal hormone receptor?

Authors:de Wied D, Elands J, Kovács G,

Journal:Proc Natl Acad Sci U S A

PubMed ID:1847526

The neurohypophyseal neuropeptides (Arg8)-vasopressin (AVP) and [pGlu4,Cyt6]AVP-(4-8) (where pGlu is pyroglutamic acid and Cyt is cystine) facilitate the retention of one-trial-learning passive avoidance behavior in rats when administered into the cerebral ventricle immediately after the learning trial. The fragment [pGlu4,Cyt6]AVP-(4-8) was considerably more effective than AVP. Oxytocin (OXT) and [pGlu4,Cyt6]OXT-(4-8) ... More

Flavivirus NS4A-induced autophagy protects cells against death and enhances virus replication.

Authors:McLean JE, Wudzinska A, Datan E, Quaglino D, Zakeri Z,

Journal:J Biol Chem

PubMed ID:21511946

Flaviviruses include the most prevalent and medically challenging viruses. Persistent infection with flaviviruses of epithelial cells and hepatocytes that do not undergo cell death is common. Here, we report that, in epithelial cells, up-regulation of autophagy following flavivirus infection markedly enhances virus replication and that one flavivirus gene, NS4A, uniquely ... More